苦豆子总碱对小鼠急性毒性试验研究  

Study on the acute toxicity of total alkaloids from Sophora alopecuroides L. in mice

作　　者：陈申[1] 刘宇[2] 强哲[1] 陶蕾[1] 王学红[2] 梁剑平[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究,甘肃兰州730050

出　　处：《中兽医医药杂志》2013年第2期34-36,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：兰州市科技计划项目(2011-1-66)

摘　　要：采用改良寇氏法测定苦豆子总碱提取物对小鼠腹腔注射的急性毒性,评价其安全性,为临床安全用药提供依据。给试验组小鼠灌服不同浓度的苦豆子总碱提取物,观察给药后小鼠的临床表现与死亡情况,采用改良寇氏公式计算LD50及LD50的95%可信限,结果表明,苦豆子总碱经腹腔注射的LD50为559.24 mg/kg,95%可信限为471.95-663.59 mg/kg。试验结果提示,苦豆子总碱的毒性较低,临床用药安全可靠。A modified Karber＇s method was used to determine the acute toxicity of total alkaloids from Sophora alopecuroides L.in mice.The experimental mice were intraperitoneally injected with different concentrations of total alkaloids,then the activities and survival of mice was observed.The acute toxic reaction was recorded and the LD50 value and 95% confidence limit of LD50 were determined by the modified Karber＇s method.The results showed that LD50 was 559.24mg/kg body weight and its 95% confidence limit was 471.95-663.59 mg/kg body weight.This demonstrated that the total alkaloids from Sophora alopecuroides L.were safe for clinical application with lower toxicity.

关 键 词：苦豆子总碱 小鼠 急性毒性 

分 类 号：S853.74[农业科学—临床兽医学]