大剂量甲泼尼龙可能加速急性百草枯中毒小鼠死亡的初步研究  

High-dose methylprednisolone could accelerate death of mice with acute paraquat poisoning: A preliminary study

作　　者：刘鹏[1] 周越塑[1] 孟庆义[2] 李跃旗[1] 赫兢[1] 何跃忠[3] 

机构地区：[1]解放军第三〇二医院急诊科,北京100039 [2]中国人民解放军总医院急诊科,北京100853 [3]军事医学科学院附属医院中毒救治中心,北京100071

出　　处：《中国工业医学杂志》2013年第5期330-331,334,共3页Chinese Journal of Industrial Medicine

摘　　要：目的观察大剂量甲泼尼龙琥珀酸钠对于急性百草枯(paraquat,PQ)中毒小鼠的疗效。方法 65只Balb/c小鼠随机分成染毒组30只、治疗组30只和甲泼尼龙对照组5只;P染毒组依据染毒不同剂量PQ(50、70、100、150、200、300 mg/kg)随机分成6组,治疗组在小鼠染毒不同剂量的PQ后均加用大剂量甲泼尼龙琥珀酸钠(195 mg/kg),甲泼尼龙对照组仅给予甲泼尼龙琥珀酸钠(195 mg/kg);比较染毒组和治疗组的半数致死量。结果(1)染毒组小鼠在染毒后24 h、48 h、72 h的LD50分别为216.67 mg/kg、216.67 mg/kg和188.24 mg/kg;治疗组小鼠在染毒后24 h、48 h、72 h的LD50分别为70.42 mg/kg、70.42 mg/kg和87.66 mg/kg。(2)治疗组小鼠染毒后24 h、48 h、72 h的LD50明显小于染毒组。结论急性PQ中毒Balb/c小鼠腹腔注射大剂量甲泼尼龙琥珀酸钠后可能加速其死亡。Objective To observe the effect of high dose of methylprednisolone on Balb/c mice with acute paraquat （PQ） poisoning. Methods Balb/e mice were randomly divided into poisoning group （30 mice）, treatment group （30 mice） , and medrol control group （5 mice）. The poisoning group and treatment group were randomly divided into 6 sub-groups according to different PQ doses （50, 70, 100, 150, 200, and 300 mg/（kg ~ d）, respectively） by i.p. injection; after reeieving the PQ injection, the mice in treatment group were immediately received 200 mg/kg of methylprednisolone i.p. injection. The con- trol mice were only received 200 mg/kg of methylprednisolone by i.p. injection. The LDs0 values between poisoning group and treatment group were compared each other. Results （ 1 ） At 24, 48 and 72 h after exposure to paraquat, the LDso values were 216. 67 mg/kg, 216. 67 mg/kg and 188. 24 mg/kg, respectively, in poisoning group, and 70. 42 mg/kg, 70. 42 mg/kg and 87. 66 mg/kg in treatment group, respectively. （2） The LD50 values in treatment group were smaller than that in poisoning group at either 24, 48 or 72 h after exposure to paraquat. Conclusion A single intraperitoneal injection of high-dose methylpred- nisolone could accelerate the death of Balb/c mice with paraquat poisoning.

关 键 词：急性中毒 百草枯(PQ) 甲泼尼龙 小鼠 

分 类 号：R139.3[医药卫生—劳动卫生]