森嘎拉草莓叶片不定芽诱导及转化体系的建立  

Studies on Induction of Adventitious Bud of Leaflet and Construction of Transformation System of 'Sengara' Strawberry

作　　者：张丙秀[1,2,3] 李柱刚[2] 赵树亮[3] 刘丹[3] 高庆玉[3] 

机构地区：[1]东北林业大学博士后科研流动站,哈尔滨150086 [2]黑龙江省农业科学院博士后科研工作站,哈尔滨150086 [3]东北农业大学,哈尔滨150030

出　　处：《吉林农业科学》2013年第5期69-72,共4页Journal of　Jilin Agricultural Sciences

基　　金：公益性行业体系专项基金项目(201103037)

摘　　要：本文以草莓森嘎拉组培苗离体叶片为外植体,研究了暗培养、不同激素配比对不定芽分化及暗培养对叶片不定芽诱导率的影响。结果表明:森嘎拉的叶片诱导最适培养基为MS+3.0 mg/L 6-BA+0.2 mg/L2,4-D,在此培养基上诱导率可达73.3%,平均出芽数可达2.2。先暗培养7 d,再进行光培养,不定芽的诱导率较高,不定芽诱导率为78.2%。对卡那霉素筛选压力和除菌剂浓度的研究表明:用300 mg/L(Cef)除菌,外植体分化率最高,并确立了森嘎拉在不定芽分化阶段的Km筛选压力为20 mg/L。An efficient regeneration system was established from the explants of leaves of ＇Sengara＇ strawberry （Fragaria ananassa Duch.）. The effects of dark incubation and plant growth regulator combinations on adventitious bud induction were studied. The results showed that the optimum medium for＇Songara＇leaves induction was MS ＋ 3.0 mg/L 6-BA ＋ 0.2 mg/L 2,4-D, and the induction rate was 73.3%. The average sprouting numbers was up to 2.2. Adventitious bud induction rate was higher when dark cultured for 7 days then the light culture, and the adventitious bud induction rate was 78.2%. For 300 mg/L Cef sterilization, explants differentiation rate was the highest, and Km screening pressure was 20 mg/L for ＇Sengala＇in adventitious bud differentiation stage.

关 键 词：草莓 不定芽 培养基 转化体系 

分 类 号：S668.401[农业科学—果树学]