HPLC-ELSD法测定积雪草药材及配方颗粒剂中积雪草苷的含量  被引量:6

Determination of asiaticoside in Centella asiatica and its formula particles by HPLC-ELSD

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作  者:曹金华[1] 谷莉[2] 吕磊[1] 李悦悦[1] 周闺臣[1] 赵亮[1] 张国庆[1] 

机构地区:[1]第二军医大学东方肝胆外科医院,上海200438 [2]第二军医大学药学院,上海200433

出  处:《药学实践杂志》2013年第5期384-385,392,共3页Journal of Pharmaceutical Practice

摘  要:目的建立高效液相一蒸发光散色器法测定积雪草药材及配方颗粒剂中积雪草苷的含量。方法采用依利特HypersilODSC18柱(4.6mm×250mm,5μm),流动相为乙腈-水(v/v:3:8),流速为0.6ml/min。结果积雪草苷在23.2-580.0μg/ml范围内呈良好的线性关系,r=0.9996;日内和日间精密度分别为2.1%和3.6%;药材的加样回收率为95.2%(RSD=3.2%,n=6),配方颗粒的加样回收率为104.6%(RSD=1.0%,n=6);5个产地的药材中积雪草苷的含量分别为0.33%、0.52%、0.22%、0.28%和0.39%,5个批号的配方颗粒中积雪草苷的含量分别为0.40%、0.44%、0.39%、0.44%和0.42%。结论该分析方法简便,结果准确,适用于积雪草药材及配方颗粒剂的质量控制。Objective To develop a method for determination of assiticoside in Centella asiatica and its formula particles. Methods Hypersil ODS Cls column was used (4.6 mm ×250mm,5μm), withacetonitrile-water(v/v = 3 : 8) as mobile phase, and a flow rate of 0.6 ml/min. Results The result showed that the calibration curve was linear in the range of 23.2 - 580.0 μg/ml for asi- aticoside, intra-day and inter-day precision results were 2.1% and 3.6% , the average recovery of asiaticoside were 95.2% ( RSD = 3. 2% , n = 6) in centella asiatica and 104.6% (RSD = 1.0% , n = 6) in its formula particles. The contents of asiaticoside in 5 medicinal materials were 0.33% ,0.52% ,0.22% ,0.28% and 0.39% ;while in 5 formula particles were 0.40% ,0.44% ,0.39% ,0.44% and 0. 42%. Conclusions The method was simple and accurate for quality control of Centella asiatica and its formula particles.

关 键 词:积雪草 积雪草苷 高效液相-蒸发光散射器 含量测定 

分 类 号:R917[医药卫生—药物分析学]

 

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