广西地区壮族 瑶族人群人类血小板抗原基因的多态性分析  

Human platelet antigen gene polymorphism analysis in Yao and Zhuang nationality in Guangxi

作　　者：杨兰[1,2] 梁秀云[1,2] 曾江辉[1,2] 

机构地区：[1]广西医科大学第三附属医院 [2]南宁市第二人民医院检验科,530031

出　　处：《检验医学与临床》2013年第20期2644-2645,2647,共3页Laboratory Medicine and Clinic

基　　金：广西壮族自治区南宁市科学研究与技术开发计划项目(201106032C)

摘　　要：目的研究广西地区壮族、瑶族人群人类血小板抗原(HPA)1～17bw基因的表达差异。方法采用序列特异引物-聚合酶链反应(PCR-SSP)方法对广西地区100例壮族和100例瑶族健康个体进行HPA 1～17bw系统基因分型,比较壮族和瑶族的HPA基因频率。结果等位基因a是广西地区壮族、瑶族健康个体主要的HPA1～17bw基因型别。在H-W平衡检验中,瑶族健康人群HPA1～17bw基因多态性中除HPA1、2、6外,其他均符合H-W群体遗传平衡法则(P>0.05);壮族健康个体中,所有HPA各系统符合H-W群体遗传平衡法则(P>0.05)。瑶族和壮族HPA系统抗原不配合率分析中,HPA3和HPA15不配合率最高,皆超过30%。比较两个民族的基因频率,发现瑶族的HPA-3和HPA-15的基因多态性明显低于壮族(χ2=12.242,P=0.002;χ2=6.209,P=0.045)。结论广西地区壮族和瑶族HPA基因多态性分布存在明显的种族差异,特别是HPA-3和HPA-15系统杂合度最高,抗原分布不配合比例相对高,在临床配合性血小板输注中必须加以重视。Objective To analyze the expression difference of human platelet antigen （HPA） 1-17bw gene be-tween Yao and Zhuang nationality in Guangxi .Methods Genotyping of HPA 1 -17bw system of 100 cases of Yao nationality and 100 cases of Zhuang nationality were conducted by using PCR-SSP ,and genotype and gene frequency were calculated .Results The alleles a was the common genotype of HPA 1-17bw gene in Zhuang and Yao national-ity of Guangxi .H-W population genetic equilibrium test indicated that ,in Yao nationality ,most gene polymorphisms of HPA 1-17bw gene ,excepting HPA1 ,2 and 6 gene polymorphisms ,were conformable to H-W population genetic e-quilibrium law （P〉0 .05） ,and in Zhuang nationality ,all HPA system were conformable to H-W rule（P〉0 .05） .A-mong all HPA system ,the mismatch rates of HPA3 and HPA15 were the highest ,more than 30% ,between Yao and Zhuang nationality .Comparison of gene frequencies between the two groups indicated that the gene polymorphisms of HPA3 and HPA15 in Yao nationality were lower than Zhuang nationality （χ2 =12 .242 ,P=0 .002 ;χ2 =6 .209 ,P=0 .045） .Conclusion There could be significant ethnic difference of HPA gene polymorphism between Zhuang and Yao nationality in Guangxi .The distribution of HPA3 and HPA15 gene frequency could be polymorphous ,and should be paid more attention in matched platelet transfusion in clinic .

关 键 词：壮族 瑶族 人类血小板抗原 基因多态性 

分 类 号：R446.62[医药卫生—诊断学]