鲍曼不动杆菌的耐药性分析及其表面生理变化  

The antibiotic resistance analysis and the surface physiological change of Acinetobacter baumanii

作　　者：李云[1] 夏蔚珉 张丽萍[2] 张文[1] 杨秀珍[1] 张彩虹[1] 

机构地区：[1]淄博市中心医院检验科,山东淄博2550362 [2]中国检验检疫科学研究院,北京100025

出　　处：《国际检验医学杂志》2013年第20期2650-2651,2655,共3页International Journal of Laboratory Medicine

基　　金：淄博市科技局基金资助项目(2011GG01222)

摘　　要：目的通过检测分析该地区鲍曼不动杆菌耐药性及生理形态变化,了解其耐药性的发展趋势。方法用Vitek2-Compact自动微生物鉴定系统检测鲍曼不动杆菌对18种抗菌药物的耐药性。用原子力显微镜比较分析其耐药菌株和敏感菌株的形态学变化。结果耐药性检测表明,鲍曼不动杆菌泛耐药现象普遍,其中对头孢孟多的耐药率达100%,重症监护室(ICU)内鲍曼不动杆菌对头孢类抗菌药物耐药性达84%。通过原子力显微镜观察,鲍曼不动杆菌耐药菌株形态发生了明显变化,菌体变成卷曲不规则,表面凹凸不平。结论鲍曼不动杆菌耐药性严重,需严格根据药物检测来指导用药,以减少耐药性变异菌的产生。Objective To investigate the drug resistance and physiological changes of local Acinetobacter baumannii,and to get a comprehensive understanding of the development trendency of drug resistance.Methods Automatic microorganism identification system was used to detect Acinetobacter baumannii and its drug-resistance to 18 antibiotics.Moreover,the atomic force microscopy was used to analyze the morphological changes of drug-resistant strains and sensitive strains.Results The detection of drug-resistance showed that the pan drug resistance of Acinetobacter baumannii was widespread,in which the cefamandole resistance rates was up to 100%.In intensive care unit（ICU）,the drug resistance rate of Acinetobacter baumannii to cephalosporin antibiotics was 84%,The result of atomic force microscopy showed that the resistant strains form of Acinetobacter baumannii changed significantly,and the bacteria became curly irregular and uneven surface.Conclusion The drug-resistance of Acinetobacter baumannii is very serious,so it is important to guide drug by the strictly drug testing,in order to reduce the resistant bacteria.

关 键 词：鲍氏不动杆菌 抗药性 微生物 原子力显微镜 重症监护室 形态变化 

分 类 号：R446.5[医药卫生—诊断学]