东北山樱桃核总黄酮成分双水相萃取条件的优化  

Optimization of the extraction of flavones from Cerasus sachalinensis Kom using Two-Phase System

作　　者：陈丽华[1] 

机构地区：[1]<北华大学学报>编辑部,吉林吉林132000

出　　处：《西北农林科技大学学报（自然科学版）》2013年第10期137-142,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：吉林省教育厅"十二五"科学技术研究项目(20120138)

摘　　要：【目的】探究聚乙二醇-硫酸铵双水相体系在东北山樱桃核总黄酮成分分离纯化中的应用,并确定最佳萃取条件。【方法】采取单因素试验,分析聚乙二醇相对分子质量、聚乙二醇-硫酸铵质量比、pH、温度对东北山樱桃核总黄酮成分得率的影响,对双水相萃取条件进行优化筛选,并对筛选出的聚乙二醇-硫酸铵双水相萃取最佳体系分离纯化东北山樱桃核总黄酮成分的效果进行检验。【结果】在聚乙二醇相对分子质量为400、m(聚乙二醇):m(硫酸铵)=1:1.5、pH为5.6、温度为50℃的条件下,东北山樱桃核总黄酮成分的得率最高,两相间平均分配系数为130.66,东北山樱桃核总黄酮的平均得率为99.95%。【结论】在最佳双水相萃取条件下,东北山樱桃核总黄酮的得率较高,两相间分配系数较大,且该方法快速简便,结果稳定可靠。【Objective】This study aimed to explore the application of polyethylene glycol ammonium sulfate aqueous two phase system in the separation and purification of total flavonoids from Cerasus sachalinensis Kom,and determine the optimal extraction conditions.【Method】Single factor exploration method was used to analyze the influence of four factors：Polyethylene glycol （PEG） molecular weight,polyethylene glycol ammonium sulfate ratio,pH value,and temperature on the receiving rate of total flavonoids of the C.sachalinensis Kom.The optimum combination was filtered out and the best results to do the verification test were selected.In addition,the verification test was used to examine the purification effect of the polyethylene glycol ammonium sulfate aqueous twophase extraction system.【Result】When PEG400 was used,PEG ammonium sulfate was 1∶1.5,pH was 5.6,and temperature was 50 ℃,the maximum extraction rate was obtained with the upper and lower phases distribution coefficient on average of 130.66 and the getting rate on average of 99.95%.【Conclusion】The best purification conditions for the receiving rate of total flavonoids from C.sachalinensis Kom and the coefficient of the two interphase distribution rate were obtained.

关 键 词：东北山樱桃核 总黄酮 分离纯化 双水相萃取 聚乙二醇 硫酸铵 

分 类 号：R284.2[医药卫生—中药学]