富半胱氨酸61在哮喘小鼠气道上皮细胞中表达的研究  

Study of the Expression of Cysteine-rich 61 Protein in Airway Epithelial Cells of Asthmatic Mice

作　　者：曹勇[1] 陈辉龙[1] 方慧娟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院呼吸与危重症医学科,武汉430030

出　　处：《医药导报》2013年第10期1274-1277,共4页Herald of Medicine

基　　金：国家自然科学基金(30900647)

摘　　要：目的探讨富半胱氨酸61(cyr61)在哮喘小鼠中的表达及氨茶碱对其影响。方法 40只雌性BALA/c小鼠随机分为哮喘组、氨茶碱干预组、哮喘对照组及正常对照组,每组10只。哮喘组通过卵清蛋白(OVA)致敏和激发的方法制作哮喘小鼠模型。氨茶碱干预组在制作哮喘模型同时,于每次激发后尾静脉注射氨茶碱干预。哮喘对照组在制作哮喘模型同时,给予0.9%氯化钠溶液替代氨茶碱干预。瑞特-吉姆萨染色分类计数支气管肺泡灌洗液(BALF)中细胞数,免疫组织化学技术检测小鼠支气管上皮组织cyr61表达,酶联免疫吸附测定(ELISA)检测BALF中白细胞介素4(IL-4)含量。结果哮喘组小鼠气道上皮细胞cyr61蛋白表达明显高于正常组小鼠(P<0.05),氨茶碱干预组小鼠气道上皮细胞中cyr61蛋白表达较哮喘组明显降低(P<0.05),但仍高于正常组(P<0.05)。cyr61蛋白表达与BALF中有核细胞总数、嗜酸性粒细胞、淋巴细胞以及IL-4呈正相关(P<0.05)。结论 cyr61蛋白可能参与了哮喘的发病过程,其异常表达可能与气道炎症有关。Objective To study the expression of cysteine-rich 61 （ cyr61 ） protein in asthmatic mice and the influence of aminophylline on it. Methods Forty BALB/e mice were divided into asthma group （ n = 10）, aminophylline group （ n = 10 ） , asthma control group （n = 10 ） and normal group （n = 10 ）. The asthma mouse model was established by ovalbumin （OVA） - sensitization and -challenge. Aminophyfline group was administered with aminophylline into the tail vein after OVA challenge. Asthma control group was administered with normal saline. The expression of eyr61 protein in the lung tissues was detected by immunohistochemistry. The level of intedeukin-4 protein was determined in the bronchoalveolar lavage fluid （BALF） by enzyme- linked immunosorbent assay （ELISA）. The count of inflammatory ceils in BALF was also analyzed. Results The results showed that the expression of cyr61 protein in asthma group was significantly higher than that in normal mice （ P〈0.05 ）. The expression level of cyr61 was significantly decreased in aminophylline group as compared with asthma group （P〈0.05）, though it was still higher than that in normal mice （P〈0.05）. The eyr61 protein expression correlated positively with the total caryocytes （P〈0.05） ,eosinophils （P〈0.05）,lymphocytes （P〈0. 05） and IL-4 level （P〈0.05） in the BALF. Conclusion Our findings suggest that cyr61 might contribute to the airway inflammation in asthma.

关 键 词：富半胱氨酸61 氨茶碱 哮喘 支气管 炎症 气道 上皮细胞 气道 

分 类 号：R965[医药卫生—药理学]