b型流感嗜血杆菌结合疫苗的制备及免疫学特性  

Prepartion and Immunological Characteristics of Haemophilus Influenza Type b Polysaccharide Protein Conjugate Vaccine

作　　者：靳志刚[1] 杨辉[1] 田玉珍[1] 谭小梅[1] 蒲江[1] 张谦[1] 沈玉霖 王永谦[1] 谢贵林[1] 王秉瑞[1] 李凤祥[2] 何莉[2] 袁曾麟[2] 

机构地区：[1]兰州生物制品研究所,兰州730046 [2]中国药品生物制品检定所,北京100050

出　　处：《中国生物制品学杂志》2000年第1期19-22,共4页Chinese Journal of Biologicals

摘　　要：目的制备ｂ型流感嗜血杆菌结合疫苗，观察免疫学特性。方法通过已二酸二肼（ＡＤＨ），将纯 化的荚膜多糖抗原与破伤风类毒素（ＴＴ）蛋白共价结合，制备ｂ型流感嗜血杆菌结合物。以２．５μｇ多糖或含２．５μｇ 多糖结合物经皮下免疫ＮＩＨ雌性小鼠，用ＥＬＩＳＡ法检测小鼠血清ＰＲＰ抗体及Ｔ抗体水平。结果用本实验方法提 取的多糖、合成的多糖衍生物、多糖－蛋白结合物都具有ｂ型流感嗜血杆菌的抗原特异性，其化学组成及结构特性 与国外文献报道结果基本一致。单独用多糖免疫小鼠未能诱导高水平的血清ＰＲＰ抗体，而用结合物免疫小鼠却诱 导出高水平的血清ＰＲＰ抗体及ＴＴ抗体，并存在免疫记忆性。结论本实验为进一步临床评价结合物的体内保护 性效果提供了实验基础。Objective To prepare Haemophilus influenza type b(Hib)polysaccharide protein conju- gate vaccine and study on the immunological characteristics of it. Methods Hib polysaccharide protein conjugate vaccine was prepared by carbodiimide-mediated coupling of adipic acid hydrazide derivtives of Hib capsular polysaccharides with tetanus toxoid(TT). Female NIH mice were subcutaneously injected with 2. 5ug of po- lysaccharide or the conjugate containing 2. 5ug of polysaccharide, and the PRP and TT antibodies in the sera of them were detected by ELISA. Results All the polysaccharide, polysaccharide derivatives and polysaccharide- protein conjugate showed the specific antigenicities of Hib, and the chemical compositions and structures of them were basically consistent with those reported abroad. Single administration of polysaccharide could not induce PRP antibody at a high level in the sera of mice, however, PRP and TT antibodies at high levels were induced in the mice injected with conjugate, and immunological memory was observed. Conclusion The study laid a foundation of further evaluation of protective effect of conjugate.

关 键 词：B型流感嗜血杆菌 结合疫苗 免疫学 

分 类 号：R378.41[医药卫生—病原生物学] R392.7[医药卫生—基础医学]