肾综合征出血热病毒Z10株在Vero细胞上传代适应和增殖动态  

Adaption and Proliferation of Hantavirus Z10 Strain in Vero Cells

作　　者：姚智慧[1] 董关木[1] 俞永新[1] 孔艳[1] 刘文雪[1] 安祺[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《中国生物制品学杂志》2000年第1期23-24,共2页Chinese Journal of Biologicals

摘　　要：目的为了肾综合征出血热病毒滴度达到规模化生产要求。方法将肾综合征出血热病毒 Ｚ１０ 株在Ｖｅｒｏ细胞上传代适应，不同时间取样，测定病毒滴度。结果从第２代即可稳定达到高滴度。转瓶培养比静 止培养的病毒峰值出现的更早，第 ４ ｄ即可达到１０８／ｍｌ以上，且可多次收液。结论初步确立了疫苗生产中病毒制 备工艺。Objective To promote the large-scale production of Hantavirus. Methods Hantavirus Z10 strain was adapted in Vero cells. The samples at differenct times were collected and detected for virus titers. Results The strain showed a stable and high titer after being undergone only 2 passages. The peak virus appeared earlier in rolling bottles than that in flasks. At the 4th day after being cultural in rolling bottles, the virus reached a titer of 108/ml and can be harvested for many times. Conclusion The procedure for preparing virus during the production of vaccine has been preliminarily established.

关 键 词：肾综合征出血热病毒 Z10株 VERO细胞 传代适应 

分 类 号：R373.32[医药卫生—病原生物学]