尖吻蝮蛇毒内一种新的抗血小板凝集蛋白agkisacutacin的纯化及其性质  

Purification and Characterization of a Platelet Agglutinating Inhibiting Protein (Agkisacutacin) from Agkistrodon acutus Venom

作　　者：承新[1] 许贞玉 刘庆都[1] 李向明[1] 李兆羿 刘兢[1] 

机构地区：[1]中国科学技术大学生命科学学院,合肥230027 [2]合肥兆峰科大药业有限公司新药研究发展部,合肥230088

出　　处：《生物化学与生物物理学报》2000年第6期653-656,共4页

摘　　要：从皖南尖吻蝮蛇毒中经阴离子交换层析和凝胶过滤层析分离纯化得到抗血小板凝集蛋白agkisacutacin ,纯化的agkisacutacin由分子量为 1 4kD和 1 5kD的 2条肽链通过二硫键连接 ,能有效抑制ristocetin诱导的血小板凝集 (IC5 0 为 1 8.5mg/L) ,能轻微抑制凝血酶诱导的血小板聚集 (IC5 0 为 1 .2 2 g/L) ,但对ADP、胶原诱导的血小板聚集无影响。agkisacutacin不具有纤溶活性、抗凝活性、磷脂酶A2 活性和出血活性 ,是一种潜在的安全。A platelet agglutinating inhibiting protein (agkisacu tacin) was isolated from the venom of Agkistrodon acutus by DEAE Sepharose F ast Flow and size exclusion chromatography. The purified product was a 29 kD pro tein composed of two disulfide bond linked polypeptide chains of molecular weig ht of 14 kD, 15 kD, respectively. It completely inhibited ristocetin induced pl atelet agglutination with an IC 50 value of 18.5 mg/L. It also inhibited th rombin induced platelet aggregation with an IC 50 value of 1.22 g/L, but i t did not affect the platelet aggregation induced by collagen and ADP. No fibrin olytic activity, phospholipase A 2 activity, anticoagulant activity, haemorrhag ic activity or lethal activity were detected in agkisacutacin. Therefore this pr otein may offer considerable therapeutic potential in treatment of platelet ric h thrombosis.

关 键 词：尖吻蝮蛇毒 抗血小板凝集 分离纯化 

分 类 号：Q959.6[生物学—动物学]