不同月份养殖草鱼幼鱼消化道微生物群落动态变化研究  

Intestinal microbiota changes of grass carp (Ctenopharyngodon idellus) in different months

作　　者：倪加加[1,2] 余育和[1] 

机构地区：[1]中国科学院水生生物研究所、中国科学院水生生物多样性与保护重点实验室,湖北武汉430072 [2]中国科学院大学,北京100049

出　　处：《水产学报》2013年第10期1558-1563,共6页Journal of Fisheries of China

基　　金：国家“九七三”重点基础研究发展计划(2009CB118705);国家自然科学基金项目(30970358,31071896)

摘　　要：为了评估草鱼消化道微生物群落结构在草鱼食性转化后的变动情况,实验对食性转换完成后的草鱼消化道微生物群落结构进行了PCR-DGGE分析。结果显示,不同月份采集草鱼消化道微生物群落结构存在明显差异(Monte Carlo检验,A:F=3.41,P=0.002;B:F=3.58,P=0.002),而被广泛认为影响宿主消化道微生物群落结构的宿主大小、丰满度等因素则在短期内并没有发现会对草鱼消化道微生物群落结构产生影响。实验表明,短期内环境因素可能是造成草鱼消化道微生物群落结构变动的主要因素。本实验结果将为深入阐述草鱼消化道微生物群落结构的变化规律、消化道益生菌的作用机理、消化道微生物群落结构变动与草鱼疾病的关系等问题积累基础材料。In order to assess the changes of the intestinal microbial community after the feeding conversion of grass carp（Ctenopharyngodon idellus）,we analyzed the intestinal microbial communities of grass carp by PCR-DGGE.The results indicated that there were significant differences among the intestinal microbial communities from the grass carps caught in different months.However,the sample size and fullness were not detected to have significant correlation with the intestinal microbial community,which were generally recognized as the factors influencing the intestinal microbial community.These results implied that environmental difference probably is the most important factor influencing the intestinal microbial community in a short-term.Our results will provide the basic information to elucidate the change rule of intestinal microbial community of grass carp,and the action mechanism of the intestinal probiotics,the relationship between intestinal microbes and the fish diseases.

关 键 词：草鱼 消化道微生物群落 变性梯度凝胶电泳 食性转换 

分 类 号：Q938.8[生物学—微生物学] S965[农业科学—水产养殖]