桥本甲状腺炎患者外周血单个核细胞上催乳素受体mRNA的表达  

Expression of prolactin receptor messenger ribonucleic acid on peripheral blood mononuclear cells in patients with Hashimoto＇s thyroiditis

作　　者：孔艳华[1] 任安[1] 杨静[1] 陈若平[1] 荆春艳[1] 陈燕[1] 郑海兰[1] 王艳燕[1] 

机构地区：[1]安徽医科大学附属省立医院内分泌科,合肥230001

出　　处：《中华内分泌代谢杂志》2013年第10期881-883,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：安徽省卫生厅重点项目基金（10021303013）

摘　　要：探讨催乳素及其受体在桥本甲状腺炎发病机制中的作用。选取新诊断桥本甲状腺炎甲状腺功能正常者33例，正常对照者32名，用荧光实时定量PCR定量检测催乳素受体mRNA，化学发光法测定血清催乳素，酶联免疫法检测白细胞介素2。与对照组相比，桥本甲状腺炎组患者外周血单个核细胞上催乳素受体mRNA含量、催乳素及白细胞介素2水平明显增高［1.190±0.913对0.149±0.130, （13.941±7.109对11.022±3.461）ng/ml, （102.165±43.716对81.862±32.128）pg/ml, P〈0.05］，提示催乳素可能通过与其受体结合参与人体免疫反应，影响细胞因子的表达，参与桥本甲状腺炎的发生发展。To investigate the role played by serum prolactin and prolactin receptor in the pathogenesis of Hashimoto′s thyroiditis（HT）. 33 newly diagnosed patients with HT with euthyroidism and 32 healthy subjects were enrolled as HT group and normal control group respectively. The expression of prolactin receptor mRNA on peripheral blood mononuclear cells （PBMC） were detected by realtime fluorescence quantitative PCR, serum prolactin was determinated by immunochemiluminescence assay and interleukin 2 was detected by Enzyme-linked immunosorbent. Compared with the controls, the level of prolactin receptor mRNA on PBMC, serum prolactin, interleukin 2 in HT patients were significantly higher[1.190±0.913 vs 0.149±0.130, （13.941±7.109 vs 11.022±3.461）ng/ml, （102.165±43.716 vs 81.862±32.128）pg/ml, P〈0.05]; it implies that prolactin may be involved in the body′s immune defences through combing with its receptor, promoting the activation of lymphocytes, and participating in the development of Hashimoto＇s thyroiditis.

关 键 词：催乳素受体 桥本甲状腺炎 逆转录聚合酶链反应 实时荧光定量PCR 

分 类 号：R581[医药卫生—内分泌]