应用3，4-DAA介导IDO高表达诱导小鼠皮肤移植免疫负向调节的研究  

Negative regulation on allograft immune response induced with up-regulated expression of IDO mediated by3,4-DAA

作　　者：黄君龄[1] 廖炯博[1] 邵琨[1] 王祥慧[1] 徐达[1] 周佩军[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院,200025

出　　处：《中华器官移植杂志》2013年第10期620-624,共5页Chinese Journal of Organ Transplantation

基　　金：上海市科委器官移植重点实验室研究项目（10Dz2212000）

摘　　要：目的探索N-3，4-二甲基氧基肉桂酰一邻氨基苯甲酸（3，4-DAA）通过吲哚胺2，3双加氧酶（IDO）高表达介导小鼠皮肤移植免疫负向调节的作用及其机制。方法建立小鼠皮肤移植模型，收集受鼠脾脏淋巴细胞及血清，检测白细胞介素2（IL-2）和7干扰素（IFN-7）含量；流式细胞仪测定T淋巴细胞各亚群；RT_PCR检测ID0信使RNA（mRNA）基因表达水平。结果3，4-DAA对小鼠脾脏淋巴细胞增殖有显著抑制作用，并呈现剂量效应关系。3，4-DAA可显著抑制小鼠IL-2和IFN-7的产生（P〈0．05）。3，4-DAA处理组CD3＋、CD4＋、CD8＋T淋巴细胞百分率呈现下降趋势，同时调节性T淋巴细胞百分率增加，但差异无统计学意义（P〉0．05）。3，4-DAA处理组ID0mRNA基因表达较对照组均显著增高（P〈0．05）。结论3，4-DAA可抑制T淋巴细胞增殖，并通过上调IDO高表达下调移植物免疫应答反应，显示出免疫负向调节的效应。Objective To investigate role of N-（30,40-dimethoxycinnamonyl） anthranilic acid （3,4-DAA） in mediating the negative immune regulation by indoleamine 2, 3 dioxygenase in mice skin transplantation. Method The mice skin transplant model was established. T lymphocytes were isolated from mice sptenocytes, and serum was collected. ELISA was applied to detect the IL-2 and IFN-7 concentrations in culture supernates of mice spleen lymphocytes and serum. Flow cytometry was applied to examine the phenotypes of T lymphocytes and RT-PCR to analyze the IDO mRNA transcription. Results T lymphocyte proliferation was significantly decreased by 3, 4-DAA and the concentrations of IL-2 and IFN-7 were apparently decreased in 3,4-DAA group as compared with those in control group. The percentages of CD3 ＋ , CD4＋ and CD8 ＋ T lymphocytes were lower, and the percentage of CD4 ＋ CD25 ＋ T lymphocytes was higher in 3,4-DAA disposal group than in control group, but there was no significant difference. IDO mRNA expression showed obvious increase in 3,4- DAA group as compared with control group. Conclusion 3, 4-DAA can up-regulate the IDO expression to induce the increasing metabolism of tryptophan, subsequently inhibiting T cell proliferation and showing specific property of negative immune regulation.

关 键 词：邻氨基苯甲酸类 双加氧酶类 免疫调节 皮肤移植 小鼠 

分 类 号：R392.1[医药卫生—免疫学]