一种基于MSO/GO的水中痕量汞离子（Ⅱ）检测方法研究  

Study on MSO/GO-based determination method for trace amount of aqueous Hg2＋

作　　者：孙涛[1] 何珏[1] 项序武[1] 洪小兰[1] 姚小艳[1] 张林志[1] 王艺芸[1] 谢晏讷[1] 吴文鹤[1] 吕建新[1] 

机构地区：[1]温州医科大学检验医学院、生命科学学院检验医学省部共建教育部重点实验室,325035

出　　处：《中华劳动卫生职业病杂志》2013年第10期783-786,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金项目（81101148）;浙江省大学生科技创新项目（2012R413007）;浙江省研究生创新科研项目（YK2008078）;温州市科技计划项目（Y20110021）

摘　　要：目的 运用优化的汞离子特异性寡核苷酸探针（mercury-specific oligonucleotide,MSO）和氧化石墨烯（graphene oxide,GO）,构建一种超高灵敏度荧光检测水中Hg2＋的纳米生物传感器.方法 将自主设计的MSO1（5＇端进行荧光基团FAM标记,标记为FAM-MSO1）和MSO2通过非共价键作用力吸附到GO的表面,实现纳米生物传感器的组装；利用特异错配结合（T-Hg2＋-T）和溶液中荧光信号的变化情况,进行Hg2＋的定量分析.结果 制备的GO厚度仅为1.4 nm;构建的Hg2＋纳米生物传感器,FAM-MSO1和MSO2的最佳浓度均为1μmol/L,0.5 g/L GO最佳用量为5μl,最低检测限为10 pmol/L,与除Hg2＋以外的其他10种金属离子交叉反应低,加入Na2S2O3再检测Hg2＋荧光恢复效率达65％.结论 基于MSO/GO的Hg2＋纳米生物传感器,具有操作简便、灵敏、特异、准确和可重复使用性等优点,可用于水中痕量Hg2＋的检测.Objective To establish a highly sensitive fluorometric nanobiosensor for determination of aqueous mercury ions （Hg2＋） using optimized mercury-specific oligonucleotide （MSO） probes and graphene oxide （GO）.Methods The nanobiosensor was assembled by attaching the self-designed MSO1 （5＇ end labeled with fluorophore carboxyfluorescein （FAM）,denoted as FAM-MSO1） and MSO2 to the surface of GO through strong non-covalent bonding forces.Upon the addition of Hg2 ＋,the formation of the T-Hg2 ＋-T configuration desorbed the FAM-MSO1 and MSO2 from the surface of GO,resulting in a restoration of the fluorescence of FAM-MSO1.Using the specific mispairing of T-Hg2 ＋-T and the changes in fluorescent signals in solutions,quantitative analysis of Hg2＋ could be performed.Results The average thickness of the prepared GO sheets was only 1.4 nm.For the Hg2＋ nanobiosensor,the optimum concentrations of FAM-MSO1 and MSO2 were both 1 μmol/L,the optimum volume of 0.5 g/L GO was 5 μL,and the limit of detection was 10 pmol/L; it had low crossreactivity with 10 other kinds of non-specific metal ions; the fluorescence recovery efficiency was up to 65％ in the re-determination of Hg2 ＋ after addition of Na2S2O3.Conclusion The MSO/GO-based nanobiosensor is convenient to operate,highly sensitive,highly specific,highly accurate,and reusable.It can be applied to determine trace amount of Hg2＋ in aqueous solutions.

关 键 词：汞离子特异性寡核苷酸探针 氧化石墨烯 纳米生物传感器 荧光检测 

分 类 号：X832[环境科学与工程—环境工程] TP212.3[自动化与计算机技术—检测技术与自动化装置]