NO对大鼠睡眠觉醒的调节  

THE REGULATION OF NO ON SLEEP WAKEFULNESS IN RATS

作　　者：张文慧[1] 孙碧英[1] 林殷利[1] 

机构地区：[1]上海第二医科大学生理教研室,上海200025

出　　处：《中国应用生理学杂志》2000年第4期339-342,共4页Chinese Journal of Applied Physiology

摘　　要：目的和方法 :通过对大鼠侧脑室微量注射NOS抑制剂L NAME及NO的前体L 精氨酸 (L Arg)观察两种物质对大鼠睡眠 觉醒的影响。结果 :注射 1mgL NAME(5 μl)后 4h觉醒 (W )明显增加 ,尤以注射后第 1～ 2h显著 ;4h慢波睡眠 (SWS)明显减少 ,该效应同样以注射后第 1～ 2h显著 ;异相睡眠 (PS)无明显变化。小剂量L NAME(0 .2mg ,5 μl)对大鼠的W、SWS、PS无明显影响 ;同样方法注射NO前体L Arg 30 0 μg/ 5 μl3～ 4h后W明显减少而SWS显著增加 ;大鼠侧脑室注射L Arg(30 0 μg/ 5 μl) +L NAME(1mg/ 5 μl)后 ,W、SWS及PS无明显变化。 结论 :L NAME通过抑制NOS使大鼠W增加 ,SWS减少 ,这一作用可被NO前体L Arg所拮抗 ,说明NO参与了机体睡眠Aim and Methods:The effects of an inhibitor of nitric oxide synthase L NAME and a precursor of NO(L arginine) on sleep wakefulness in rats were examined. Results: ① The time of waking was increased( P <0.01) and the time of SWS was decreased( P <0.01) during the first two hours after injection intracerebroventricularly(i.c.v.) of L NAME 1 mg,this effect was showed across the 4 h recording period( P <0.05).The i.c.v. injection of L NAME 0.2 mg had no significant effect.② The time of waking was su ppressed during the third and fourth hours after i.c.v.injection of L arginine 300 μg( P <0.05).At the same time the increase of SWS was observed ( P <0.01).③There was no change on W,SWS,PS when L NAME 1 mg was given after administration of L arginine.Conclusion: The excitatory effect of L NAME on wakefulness is mediated by inhibition of NOS.This effect could be antagonized by L arginine .It suggests that NO is involved in regulation of sleep wakefulness.

关 键 词：侧脑室微量注射 L-NAME L-精氨酸 睡眠-觉醒 NO 

分 类 号：Q428[生物学—神经生物学] R338.63[生物学—生理学]