雌酚酮体外影响骨生长的形态学研究  

MORPHOMETRIC STUDY ON THE EFFECTS OF ESTRONE ON BONE IN VITRO

作　　者：李灵芝[1] 张永亮[2] 马丽英[3] 冯凯琳[4] 

机构地区：[1]武警医学院药学教研室,天津300162 [2]武警医学院法医学教研室 [3]新疆医科大学,乌鲁木齐830054 [4]武警医学院放射教研室

出　　处：《中国应用生理学杂志》2000年第4期361-364,共4页Chinese Journal of Applied Physiology

摘　　要：目的 :从组织形态学角度综合考察雌酚酮在体外对骨吸收和骨形成的影响。方法 :16d胎龄雌性小鼠尺骨在含有雌酚酮的BGJb培养基中旋转培养 48h后 ,测量骨总长和骨干长度 ;H E染色 ,光镜下计数破骨细胞和肥大软骨细胞。结果 :当雌酚酮浓度为 10 7mol/L时 ,长骨总长和骨干长均比对照组显著增加 ;破骨细胞减少 ,肥大软骨细胞增加。结论 :雌酚酮在体外可抑制骨吸收 。Aim: To study the effects of estrone on the bone resorption and bone formation in vitro. Methods: Long bones from 16 day old female mouse fetuses were cultured in BGJb medium for 48 h.The bones were harvested, then bones length and length of their diaphyses were measured under stereo microscope. The histomorphormetric analyses on midlongitudinal sections of bones were performed. The numbers of osteoclasts and hypertrophic chondrocytes were observed under biological microscope.Results:Compared with control,there were significant increase in bones length and length of their diaphyses after treated with 10 -7 mol/L estrone.Under this condition,decreased osteoclasts and increased hypertrophic chondrocytes were observed,too.Conclusion:Estrone stimulates bone formation and inhibites bone resorption in vitro.

关 键 词：雌酚酮 长骨 骨吸收 骨形成 骨形态学 

分 类 号：R336[医药卫生—人体生理学] Q579.13[医药卫生—基础医学]