猪乙型脑炎病毒SC-YA株部分生物学性状的研究  

Biological characteristics of Japanese encephalitis virus strain from Sichuan Ya'an

作　　者：廖珊[1,2] 刘骁[1] 朱玲[1,3] 郭万柱[1] 

机构地区：[1]四川农业大学动物生物技术中心,四川雅安625014 [2]中江县畜牧局,四川中江618100 [3]四川农业大学动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国兽医科学》2013年第10期1006-1010,共5页Chinese Veterinary Science

基　　金：教育部新世纪优秀人才支撑计划项目(NCET 11-1059);四川省科技支撑计划项目(2012NZ0001)

摘　　要：为猪乙型脑炎疫苗的生产和保存提供指导和依据,本试验对乙型脑炎病毒(JEV)SC-YA株的理化特性、细胞培养特性进行了研究。理化鉴定结果表明,JEV SC-YA株是具有囊膜的RNA病毒;胰蛋白酶、酸性、高温等均可使其失去活性。该株病毒的最佳培养条件为培养液用量为6mL,接种剂量为10-4TCID50/0.1mL时,接种后第48小时病毒滴度达到最大值。结果表明,JEV SC-YA株能被用于疫苗生产。To provide guidance for the development and preservation of vaccines against Japanese encephalitis virus（JEV） in this study,we studied the property in cell culture,and physicochemical characters in BHK21 cells of JEV SC-YA strain. In result,the physical and chemical identification indicated that JEV SC-YA stain was RNA virus with capsular, which lost its activities under the conditions of trypsin, acid and high temperature. The optimal culture conditions of the JEV SC-YA strain：when the medium amount was 6 mL and the inoculation doses was 10.4 TCIDs0/0.1 mL,the virus titer reached the maximum at hour 48 post-inoculation. In conclusion,JEV SC-YA strain may be used for vaccine production.

关 键 词：猪 乙型脑炎病毒 培养特性 理化性质 

分 类 号：S852.651[农业科学—基础兽医学]