电化学技术研究铁蛋白接受电子的能力  

STUDIES ON CAPACITY OF ACCEPTING ELECTRONS FROM FASHLIVER FERRITIN WITH ELECTROCHEMICAL TECHNOLOGY

作　　者：黄河清[1] 林庆梅[2] 吴楠 李雪松 

机构地区：[1]厦门大学生命科学学院生物学系,福建厦门361005 [2]厦门大学固体表面物理化学国家重点实验室 [3]固体表面物理化学国家重点实验室,福建厦门361005

出　　处：《生物物理学报》2000年第4期680-686,共7页Acta Biophysica Sinica

基　　金：国家自然科学基金!资助项目(No.49876027);教育部高等学校骨干教师资助计划资助

摘　　要：鱼工鱼肝脏铁蛋白(LiverFerritinofDasyatisAkajei,DALF)利用自身的电子隧道直接从铂金电极上获得还原电子且用于释放铁反应。血红素不仅能络合于DALF ,形成DALF -heme分子 (DALFH),并构建成电子隧道 -血红素结构,而且加速DALFH 从铂金电极上接受电子的速率,从而达到提高释放铁速率的效果。用抗坏血酸作为还原剂时,DALF和DALFH 释放铁速率几乎相同。在有氧环境下,DALF仍然可从铂金电极上获得还原电子且加速释放铁的速率,因而认为DALF蛋白壳上存在着两种不同氧化还原电位的电子隧道。Through its electron tunnelling, liver Ferritin of Dasyatis Akajei(DALF) directly picked the reduction electrons up from the platinum electrode for releasing iron. The heme not only bound to the ferritin to constitute a molecule of DALF-heme, called DALFH and to form a structure of electron-tunnel-heme, but also accelerated the rate that DALF received the electron from the electrode for increasing rate of iron release. Using a reducer of ascorbic acid, similar rates of iron release from both DALFH and DALF were observed. DALF was shown not only to obtaining the reduction electrons but also in accelerating the rate of iron release under aerobic environments, suggesting that the protein shell in the ferritin has two electron tunnels with different redox potentials.

关 键 词：鱼肝铁蛋白 电子隧道功能 电化学特性 动力学 

分 类 号：Q592.1[生物学—生物化学] Q617