肉毒梭菌培养液澄清过滤工艺的优化  

Optimization of clarifying filtration procedure for Clostridium botulinum culture

作　　者：高建军[1] 李育合[1] 孟祥玉[1] 曹馨匀[1] 武毅[1] 刘伟涛[1] 谢小荣[1] 窦强[1] 

机构地区：[1]兰州生物制品研究所有限责任公司免疫室,甘肃兰州730046

出　　处：《中国生物制品学杂志》2013年第10期1498-1501,共4页Chinese Journal of Biologicals

摘　　要：目的优化肉毒梭菌培养液的澄清过滤工艺,并对过滤效果进行验证。方法选择不同型号的过滤器组合对A型肉毒梭菌培养液进行一级、二级澄清过滤,筛选最佳过滤器组合;用最佳过滤器组合对扩大培养批量的A、B、E、F型肉毒梭菌培养液进行澄清过滤。结果 P700K+50P过滤器组合对A型肉毒梭菌培养液进行一级、二级澄清过滤的效果最佳;用该过滤器组合对扩大培养批量的A、B、E、F型肉毒梭菌培养液进行澄清过滤,各型均可达到预期的试验目标。结论筛选出P700K+50P为A、B、E、F型肉毒梭菌培养液澄清过滤的最佳过滤器组合,为肉毒梭菌培养液澄清过滤工艺的进一步优化提供了实验依据。Objective To optimize the clarifying filtration procedure for Clostridium botulinum culture. Methods Cl. bo- tulinum type A culture was subjected to clarifying filtrations of grades 1 and 2 by combination of various types of filters, based on which the optimal combination was screened. The Cl. botulinum types A, B, E and F in scale-up culture was subjected to clarifying filtration by the optimal combination. Results The combination of P700K ＋ 50P used for clarifying filtrations of grades 1 and 2 of C1. butulinum type A showed satisfactory effect. Expected results were observed in the Cl. botulinum types A, B, E and F in scale-up culture after clarifying filtration by the optimal combination. Conclusion PT00K ＋ 50P was screened as the optimal combination for clarifying filtration of Cl. botulinum types A, B, E and F, which provided an experimental basis for further optimization of clarifying filtration procedure for C1. botulinum culture.

关 键 词：肉毒梭菌 培养液 澄清过滤 优化 

分 类 号：R378.83[医药卫生—病原生物学] R392-33[医药卫生—基础医学]