大鼠45SRNAs的cDNA克隆与序列分析  

Cloning and Analysis of cDNA Sequences of 4.5S RNAs of Rat Cells

作　　者：徐来祥[1,2] 马建章 汪永庆[3] 

机构地区：[1]东北林业大学野生动物资源学院 [2]中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室北京100080 [3]中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室

出　　处：《动物学杂志》2000年第6期24-28,共5页Chinese Journal of Zoology

基　　金：国家自然科学基金!(No.39730 0 90 )

摘　　要：利用RT PCR方法 ,首次从大鼠肝脏细胞总RNA中扩增出 4 .5SRNAs的cDNA。该cDNA被克隆到pGEM3Zf( +)质粒上 ,经酶切电泳鉴定 ,然后测序。与报道的小鼠和仓鼠 4 .5SRNAs序列进行了比较研究 。The cDNA of 4.5S RNAs were amplified by reverse transcription\|polymerase chain reaction (RT\|PCR) method using total RNA extracted from the liver tissue of rat as a template. The cDNA fragment was inserted into pGEM3Zf (+) plasmid and cloned to obtain the recombinant pGEM3Zf (+)\|4.5S.Using enzyme digestion and polyacrylamide gel electrophoresis methods, the recombinant plasmids that contained 4.5S cDNA sequences were detected. Then the cDNA sequences of rat 4.5S RNAs were sequenced, and the results were compared with 4.5S RNAs sequence of reported mouse and hamster to find its differences and analyzed the structural characters.

关 键 词：大鼠 4.5SRNAs RT-PCR 

分 类 号：Q959.837[生物学—动物学] Q953