兰属植物目标起始密码子(SCoT)遗传多样性分析  

Application of SCoT Molecular Marker in Genetic Diversity of Cymbidium

作　　者：高岭[1] 冯尚国[1] 何仁锋[1] 赵妙玉 王慧中[1] 

机构地区：[1]杭州师范大学生命与环境科学学院、浙江省药用植物种质改良和质量监控重点实验室,杭州310036

出　　处：《园艺学报》2013年第10期2026-2032,共7页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31070298,30870180);浙江省科技计划项目(2008C12081);浙江省自然科学基金项目(LQ13H280006);国家级大学生创业创新训练计划项目(201210346002)

摘　　要：应用SCoT分子标记技术对兰属14个种的24个样品进行遗传多样性分析,27条引物共扩增出259条DNA条带,其中多态性条带224条,多态性百分比达86.49%,条带大小为100～2 000 bp,多数条带分布在500～1 300 bp之间。根据SCoT标记计算的遗传距离系数得到的聚类结果可知,24种兰属植物可以分为5类:A类:春兰、建兰、蕙兰、莲瓣兰;B类:墨兰、寒兰;C类:春剑;D类:兔耳兰;E类:多花兰、文山红柱兰、虎头兰、象牙白、碧玉兰、黄蝉兰,这与传统兰属分类有所区别。In this research, SCoT markers were applied to detect the genetic diversity among 24 Cymbidum samples of 14 species. Two hundred and fifty-nine DNA bands were generated by 27 primers including 224 polymorphic bands. The polymorphic percent is 86.49%. Strip size was from 100 bp to 2 000 bp, and most of the bands were concentrated between 500 bp and 1 300 bp. Dendrograms generated from coefficient of genetic distance between species on the basis of SCoT markers showed that the germplasms were divided into 5 clusters. Cluster A was composed of C. georingii, C. ensifolium, C. faberi, and C. tortosepalum; Cluster B included C. sinese and C. kanran; C. goeringii var. ongibracteatum was cluster C; C. lancifolium belonged to cluster D; Cluster E was composed of C. floribundum, C. wenshanense, C. hookerianum, C. maguanense, C. lowianum and C. iridioides. The research showed that part of the result was different from the traditional classification of Cymbidium.

关 键 词：兰属植物 分子标记 SCoT 遗传多样性 

分 类 号：S682.31[农业科学—观赏园艺]