鹤顶兰四倍体植株的诱导与鉴定  

Induction and Determination of Tetraploid Plants of Phaius tankervilleae

作　　者：李豆豆[1] 刘芝龙[2] 黄明忠[2] 陈积天 唐燕琼[1] 杨光穗[2] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室海南大学农学院,海口570228 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《园艺学报》2013年第10期2033-2038,共6页Acta Horticulturae Sinica

基　　金：教育部热带作物新品种选育工程中心与作物学重点学科联合资助项目(lhxm-2012-7);海南省自然科学基金项目(313032);公益性行业(农业)科研专项经费项目(201203071)

摘　　要：以鹤顶兰原球茎为材料,使用秋水仙素进行了四倍体的离体诱导。结果表明,采用秋水仙素浸泡法可以诱导鹤顶兰原球茎发生倍性变化,含0.02%秋水仙素的"花宝一号"溶液浸泡处理6 d诱导率最高,达22.5%。细胞学鉴定发现,诱变成活植株中有3株为四倍体,其植株形态、气孔大小、气孔密度与二倍体存在差异,细胞核明显增大。Tetraploids ofPhaius tankervilleae were induced through the treatment ofprotocorms with colchicine in vitro. The results showed that ploidy levels of protocorms changed with colchicine soaking and the highest induction rate of 22.5% was obtained by immersing protocorms into 0.02% colchicine solution for 6 days. Cytological determination of regenerated plants indicated that three tetraploid plantlets were obtained among mutated plants survived, and the morphology, the number and size of stomata of the mutants were different from those of the diploid plants, with obviously bigger nucleus.

关 键 词：鹤顶兰 秋水仙素 四倍体 原球茎 

分 类 号：S682.31[农业科学—观赏园艺]