肿瘤抗原MUC1调节骨髓来源抑制细胞的产生  

Tumor antigen MUC1 inhibits the generation of myeloid-derived suppressor cell

作　　者：马吉春[1] 林江[1] 姚冬明[2] 邓兆群[1] 钱炜[1] 钱军[3] 陈芹[1] 杨静[3] 李云[3] 陈星星[3] 闻向梅[1] 郭竑[1] 

机构地区：[1]江苏大学附属人民医院中心实验室,镇江212002 [2]江苏大学附属人民医院检验科,镇江212002 [3]江苏大学附属人民医院血液科,镇江212002

出　　处：《免疫学杂志》2013年第11期970-974,共5页Immunological Journal

基　　金：镇江市科技支撑-社会发展项目(SH2012033);江苏大学医学临床科技发展基金项目(JLY20120012);国家自然基金(81172592;81270630);镇江市科技基础设施计划项目(SS2012003)

摘　　要：目的探讨MUC1对骨髓来源抑制细胞(MDSC)产生的影响。方法将过表达MUC1细胞(B16-MUC1)和对照细胞(B16-neo),接种于C57BL/6小鼠皮下,待肿瘤形成后,取骨髓、外周血、腹腔液和脾,通过流式细胞术检测MDSC细胞数量;分离C57BL/6小鼠股骨骨髓细胞,分别与B16-MUC1和B16-neo细胞共同培养24 h和48 h,通过流式细胞术检测MDSC细胞;采用Transwell小室法分析MUC1对MDSC细胞的趋化作用;流式细胞术检测MDSC细胞中MUC1蛋白表达。结果与接种B16-neo组C57BL/6小鼠相比,B16-MUC1细胞接种组小鼠在腹腔液和外周血中CD11b+GR-1+MDSC细胞含量明显降低(P<0.05);正常C57BL/6小鼠骨髓细胞分别与B16-neo和B16-MUC1细胞分别共同培养24 h和48 h后,与B16-neo细胞共同培养组相比,B16-MUC1细胞共同培养组的CD11b+GR-1+MDSC含量明显减少,具有统计学意义(P<0.01);另外,与B16-neo培养组相比,B16-MUC1细胞共同培养组迁移至滤膜下的MDSC细胞数量增加(P<0.05);骨髓细胞与B16-MUC1细胞共同培养组的MDSC细胞中,MUC1的表达增高。结论 MUC1能够抑制骨髓来源抑制细胞(MDSC)的产生,参与MDSC的募集,并诱导MDSC自身表达MUC1蛋白。To analysis the effect of MUC1 on myeloid-derived suppressor ce11 （MDSC）, MUC1 over-expressed ce11 BI6-MUC1 and control ce11 B16-neo were inject to the subcutaneous of C57BL/6 mice. When tumor formed, the myeloid, peripheral blood, peritoneal fluid and spleen were separated and the number of MDSC was detected by FCM. Furthermore, marrow ce11 in thighbone was separated and co-cultured with B16-MUC1 or B16-neo ce11 for 24 h and 48 h, then the number of MDSC was detected by FCM. Also chemotaxis effect of MUC1 on MDSC was analyzed by transwe11 assay, and MUC1 expression in MDSC ce11 was detected by FCM. We found that the number of CD11b＋GR-1＋ MDSC in peritoneal fluid and peripheral blood in B16-MUC1 injected group was reduced obviously, compared with B16-neo injected group （P〈 0.05）; in vitro, the number of CD1 lb＋GR-1＋ MDSC in B16- MUC1 co-cultured group was reduced in both 24 h and 48 h group, compared with B16-neo co-cultured group （P〈 0.0l）. Moreover, we found more CD11b＋GR-1＋ MDSC migrated to the membrane in B16-MUC1 co-cultured group as compared with B16-neo group （P〈 0.05）; the expression of MUC1 in MDSC ce11, which co-cultured with B16- MUC 1, was higher compared with that co-cultured with B 16-neo. In conclusion, MUC 1 may inhibit the generation of MDSC, take part in the recruitment of MDSC, and induce the expression of MUC 1 protein on MDSC itself.

关 键 词：MUC1 MDSC 免疫调节 肿瘤 

分 类 号：R392.1[医药卫生—免疫学]