姜黄素对纳米二氧化硅诱导HaCaT细胞氧化应激损伤保护作用研究  

Protective effects of curcumin on oxidative-stress-related enzyme activity in HaCaT cells induced by SiO_2 nanoparticle

作　　者：洪文旭[1,2] 陈晓丹[1,3] 彭圣明[2] 张航[1] 杨细飞[1] 刘建军[1] 

机构地区：[1]深圳市疾病预防控制中心深圳市现代毒理学重点实验室,广东深圳518055 [2]湘潭大学化学学院,湖南湘潭411105 [3]广东药学院公共卫生学系,广东广州510006

出　　处：《热带医学杂志》2013年第9期1061-1064,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(21102094);广东省医学科研基金(A2012577;B2011286);深圳市重点实验室提升项目(CXB201005260068A);深圳市科技计划项目(201102100)

摘　　要：目的研究姜黄素对纳米二氧化硅(nm-SiO2)诱导人永生化表皮(HaCaT)细胞氧化损伤相关指标的改变及保护作用。方法通过CCK-8法检测HaCaT细胞在15 nm SiO2和姜黄素作用下的细胞活力水平,测定在姜黄素干预下HaCaT细胞内活性氧簇(ROS)水平和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的酶活力水平。结果姜黄素的干预能明显改善15 nm SiO2处理引起的HaCaT细胞的氧化损伤。与SiO2组相比,姜黄素干预组能有效提高细胞存活率、降低细胞内ROS的积累;另外,姜黄素干预可以降低两个氧化应激相关的酶SOD和CAT的酶活力水平。结论姜黄素干预对由于nm-SiO2引起的HaCaT细胞的氧化应激损伤具有一定的保护作用。Objective To explore the protective effects of curcumin on reactive oxygen species （ROS） levels and antioxidant enzymes activity of HaCaT cells induced by SiO2 nanoparticles. Methods The cell viability was determined by CCK-8 method. The alteration of reactive oxygen species （ROS） levels, superoxide dismutase （SOD） activity and catalase （CAT） activity were analyzed by the corresponding assay kits. Results The injury of HaCaT cells induced by 15 nm SiO2 could be reduced by curcumin. Compared with the SiO2 group, curcumin could improve the survival rate and decreased the ROS levels. Compared with SiO2 group, both enzyme activity of SOD and CAT were significantly decreased in curcumin-treated groups. Conclusion Curcumin has significantly protective effects on the oxidative stress of HaCaT cells induced by 15 nm SiO2.

关 键 词：纳米二氧化硅 姜黄素 氧化应激 HACAT细胞 

分 类 号：R994[医药卫生—毒理学]