人脂肪间充质干细胞对皮肤创伤修复的作用  

Effects of human adipose-derived mesenchymal stem cells on cutaneous wound healing

作　　者：刘晓玉[1] 王瑞[1] 张涛[1] 庞希宁[1] 施萍[2] 

机构地区：[1]中国医科大学 卫生部细胞生物学重点实验室 干细胞与再生医学研究室,辽宁沈阳110001 [2]中国医科大学附属第一医院全科医学教研室,辽宁沈阳110001

出　　处：《基础医学与临床》2013年第11期1377-1381,共5页Basic and Clinical Medicine

基　　金：国家重点基础研究发展计划(2012CB518103);辽宁省科学技术计划(2012225080);沈阳市科学技术计划(F11-262-9-01)

摘　　要：目的探讨人脂肪间充质干细胞（hADSCs）皮内移植对小鼠皮肤创面修复的作用，为临床皮肤创伤后修复提供新的细胞治疗方法奠定基础。方法分离培养hADSCs，取3～5代细胞通过免疫荧光法检测间充质干细胞相关的细胞表面标志物CID0、CD105、CD34和CD45的表达，并分别置于骨、软骨和脂肪诱导分化培养基中进行诱导分化，检测其多向分化潜能。在实验小鼠背部制备一个面积为1cm×1 cm的皮肤创面，将磁性纳米颗粒标记的hADSCs以皮内注射方式移植到小鼠创面四周。分别于伤后0、7和14d观察创面愈合情况，并与对照组进行比较分析。结果人脂肪间充质干细胞表达CD90和CD105，不表达CD34和CD45，具有骨，软骨，脂肪诱导分化潜能；实验小鼠皮肤创伤后3d开始，可见各组创面逐渐缩小，伤后21d创面愈合。移植组于创伤后第7天创面愈合率为（74．6％±4．1％），14d为（96．5％±1．5％），均显著高于对照组7d的（26．7％±1．9％），14d的（47．3％±2．3％），（P〈0．05）。结论hADSCs移植能够提高小鼠皮肤创面愈合的速度。Objective To explore the effects of adipose derived mesenchymal stem cells （hADSCs）on cutaneous wound healing and provide a new method for clinical skin repair in the future. Methods The hADSCs were isolated by mechanical separation and enzymatic digestion. The expression profiles of CD90, CD105, CD34 and CD45 were ex- amined by immunofluorescence. The hADSCs from passage 3 - 5 were used for bone, osteogenic, adipogenic differ- entiation assays. The mice were anesthetized and a 1 cm×1 cm full-thickness excisional skin wound was made on the back. After labeling with superparamagnetic iron oxide dermally around the wound at four different injection sites. and performed to evaluate the quality of wound healing nanoparticles （SPIONs）, hADSCs were injected intra- After 0,7 and 14 d, the area of the wounds were meas- and compare with control. Results The morphologicalfeature of hADSCs displayed fibroblast-like phenotype. The cells were positive for stem cells markers, including CD90 and CD105 ,and negative for CD34 and CIM5 ,as shown by immunofluorescence. Osteogenic, chondrogenic, adipogenic differentiations were revealed in hADSCs when cultured in specific medium. The first 3 days of the ex- perimental mice in each group after skin wound, wound size gradually reduced, most of the wounds healed after 21 d. Compared with the control group （26. 7%±1.9% ）at 7 d and（47.3% ±2. 3% ）at 14 d, the most obvious effect at 7 d （74. 6% ± 4. 1% ） and 14 d （96. 5 % ± 1.5 % ） after transplantation （ P 〈 0. 05 ）. Conclusions hADM- SCs transplantation can improve wound healing.

关 键 词：脂肪 间充质干细胞 创伤愈合 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]