第三组代谢型谷氨酸受体亚型对神经病理性痛的影响  

Effects of group Ⅲ metabotropic glutamate receptors subtypes on neuropathic pain

作　　者：陈锐[1] 王华[1] 周全红[1] 江伟[1] 

机构地区：[1]上海交通大学附属第六人民医院麻醉科,上海200233

出　　处：《上海交通大学学报（医学版）》2013年第10期1328-1332,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金青年科学基金项目(81100825)~~

摘　　要：目的探讨第三组代谢型谷氨酸受体(mGluRs)亚型对神经病理性痛的调节作用。方法取30只SD大鼠,随机分为5组(n=6),于鞘内置管术后第5天分别给予单次鞘内注射等容量(10μL)生理盐水、L(+)-2-氨基-4-膦酰基丁酸(L-AP4)、VU0155041、AMN082和(S)-3,4-二甲酸苯基甘氨酸(DCPG),注药后每隔15 min和30 min分别测量大鼠50%机械缩足阈值(50%PWT)和缩足潜伏期(PWL)。另取30只SD大鼠,随机分为5组(n=6),鞘内置管并行脊神经根结扎(SNL),术后第7天给予单次鞘内注射生理盐水及同上药物10μL,注药后同法测量大鼠50%PWT和PWL。再取18只SD大鼠,随机分为空白对照组、SNL组、SNL+注药组(生理盐水、VU0155041、AMN082和DCPG)(n=3),注药组于30 min后与其余各组同取腰膨大处脊髓,Western blotting检测mGluR4、mGluR7和mGluR8的表达。结果 L-AP4、VU0155041、AMN082和DCPG对正常大鼠的痛域无影响(P>0.05);但对神经病理性痛大鼠有不同程度的镇痛作用(P<0.05),其中以VU0155041效果最为显著。与空白对照组相比,SNL组手术同侧脊髓只有mGluR4表达水平降低(P<0.05);与生理盐水组相比,其他注药组只有mGluR4表达水平升高(P<0.05);而mGluR7在各组中的表达水平差异无统计学意义(P>0.05),mGluR8在脊髓基本不表达。结论第三组mGluRs中主要是mGluR4对神经病理性痛有调节作用。Objective To investigate the effects of group ~I metabotropic glutamate receptors（mGluRs） subtypes on neuropathic pain. Methods Thirty SD rats were randomly divided into five groups （ n = 6）, and received a single intrathecal injection of 10 /xL saline, L-（＋） -2-Amino-4-phosphonobutyric acid （L-AP4）, VU0155041, AMN082 and （S） - 3,4-Dicarboxyphenylglycine （DCPG） respectively on the fifth day after intrathecal catheterization. Values of 50% paw withdrawal threshold （50% PWT） and paw withdrawal latency （PWL） were measured every 15 min and 30 min after drugs administration respectively. Another thirty SD rats were randomly divided into five groups （ n = 6）, and received a single intrathecal injection of 10 ~L saline and ditto drugs respectively on the seventh day after spinal nerve ligation （SNL） surgery and intrathecal catheterization. Values of 50% PWT and PWL were measured as the ditto methods after drugs administration. Another eighteen SD rats were randomly divided into blank control group, SNL group and SNL ＋ injection group（ saline, VU0155041, AMN082 and DCPG） （ n = 3）, all rats were taken the spinal cord at the lumbar enlargement 30 min after injection, and the expression of mGluR4, mGluR7 and mGluR8 was determined by Western blotting. Results L-AP4, VU0155041, AMN082 and DCPG had no influence on the normal rats （P 〉0. 05）, while had different analgesic effects on rats with neuropathic pain （P 〈0.05）, in which VU0155041 had the most significant effect. The expression of mGluR4 in spinal cord in SNL group was significantly lower than that in blank control group （P 〈 0. 05）, and the expression of mGluR4 in injection groups was significantly higher than that in saline group （P 〈 0.05）. There was no significant difference in the expression of mGluR7 among groups （ P 〉 0.05）, and mGluR8 was hardly expressed in the spinal cord. Conclusion mGluR4 is the main subtype of group m mGluRs involved in the regulation of neuropathic pain.

关 键 词：第三组代谢型谷氨酸受体 4型代谢型谷氨酸受体 神经病理性痛 

分 类 号：R614[医药卫生—麻醉学]