胰岛A细胞及白细胞介素-5阳性细胞在I型糖尿病小鼠胰岛表达的改变  

Change of the expression of islet A cells and interleukin-5 positive cells in pancreatic islet of mice with type 1 diabetes mellitus

作　　者：张璞[1] 梁文妹[1] 

机构地区：[1]贵阳医学院组织学与胚胎学教研室,贵阳550004

出　　处：《解剖学杂志》2013年第5期888-891,F0003,共5页Chinese Journal of Anatomy

基　　金：贵州省国际合作计划项目（黔科合外G字[2010]7017号）;贵阳市科技局大学生创业基金资助项目（筑科计[2012]08号）

摘　　要：目的：探讨I型糖尿病小鼠胰岛A细胞、白细胞介素-5（IL-5）阳性细胞的表达以及A细胞与IL-5阳性细胞的相互关系，为进一步探讨I型糖尿病发病机制及治疗方法提供依据。方法：模型诱导组采用连续多次小剂量链脲佐菌素给药法。于注射后第3、7、10、14、21及28天测空腹血糖、取胰组织，免疫组织化学SAI记检测胰。结果：与正常组及盐水对照组比较，小鼠空腹血糖水平从实验组14d时开始明显升高，以28d时最高。实验组胰岛面积有所减小。A细胞在实验组7、21和28d免疫显色强度有所减弱，平均灰度值显著升高，但7d以后细胞面数密度（NA）加大，与正常及盐水对照组比较差异有统计学意义。IL-5阳性细胞在实验3及7d免疫显色强度有所减弱，平均灰度值显著升高，其余时间点无明显变化。IL-5阳性细胞的Nn及分布的变化与A细胞的基本一致。胰岛中多数IL-5阳性细胞与A细胞位置相同。结论：I型糖尿病发生时，A细胞表达虽有所减弱，但数量增多。IL-5表达上调，定位于胰岛A细胞。胰高血糖素和IL-5参与了I型糖尿病的过程。Objective： To explore the expression of islet A cells and interleukin-5 （IL-5） positive cells in pancreatic islet of mice with type 1 diabetes mellitus （T1DM） and the relation between islet A cells and IL-5 positive cells, so as to provide the evidences for further researches on the patbogenesis and therapeutic method of TIDIVL Methods： Normal C57BL/6J mice were randomly divided into an experiment group （EG）, a saline control group （SCG） and a normal control group （NCG）. Mouse models of TIDM was induced through injecting multiple low-dose of streptozotocin. Fasting blood-glucose was measured and the pancreatic glands were taken subsequently on the 3rd, 7th, 10＇h, 14＇h, 21st and 28th days after the first injection. The immunohistochemical SABC staining method, immunohistochemical staining technique on adjacent section, image analysis were used. Results： As compared with the NCG and SCG, the fasting blood-glucose of mice injected with streptozotocin was gradually increasing from the 14th day onwards, and with the highest value on the 28th day. The area of the pancreatic islet of the model mice was reduced. The immune staining intensity of islet A cells was declined on the 7th, 21st, 28th days in EG, and the mean gray value was increased at the same time, but the numerical density on area was apparently increased in EG from the 7＇h day onwards and islet A cells were not only distributed in the periphery of the islets, but also in the middle of the islets. The immunoreaction of IL-5 positive cells was declined,on the 3ra, 7th days in EG, and the mean grey value was increased, and there was no obvious change in the other days. The changes of NA and distribution mode of IL-5 positive cells were similar to islet A cells. And the location of almost IL-5 positive cells in the pancreatic islet was the same to islet A cells. Conclusion： Although the expression of A cells is declined, the number of A cells is increased in T1DM mice. The expression of IL-5 is up-regulated, and IL-5 locates in

关 键 词：链脲佐菌素 I型糖尿病 胰高血糖素 白细胞介素-5 免疫组织化学 C57BL 6J小鼠 

分 类 号：R562.25[医药卫生—呼吸系统]