黄瓜矮生基因(cp)连锁的简单重复序列(SSRs)和序列标签位点(STS)标记  

The Simple Sequence Repeat(SSR) and Sequence-tagged Sites(STS) Markers Linked to the Compact Gene(cp) in Cucumber(Cucumis sativus L.)

作　　者：雍建朋[1] 李玉红[1] 孟永娇[1] 钟艳花[1] 程智慧[1] 陈鹏[2] 

机构地区：[1]西北农林科技大学园艺学院,杨凌712100 [2]西北农林科技大学生命学院,杨凌712100

出　　处：《农业生物技术学报》2013年第10期1152-1158,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.31171955);西北农林科技大学基本科研业务费专项资金(No.YQ2013003)

摘　　要：矮化株型是黄瓜(Cucumis sativus L.)重要的农艺性状,对于实现黄瓜的密植高效栽培和机械采收具有潜在的应用价值。矮生基因(compact gene,cp)控制着黄瓜的矮生性状,初始精细定位已经将cp基因定位在220 kb的区间,且与细胞分裂素氧化酶基因(CKX)共分离。为了进一步缩短cp基因的定位区间,本研究在原有研究基础上,以黄瓜蔓生材料WI7200(长节间)与矮生材料WI7201(短节间)为亲本,即与cp初始精细定位的F2群体(1 273株)的相同亲本,新构建了一个较大的F2群体,利用cp初始精细定位的两侧翼微卫星标记UW084680和UW084870筛选以相同亲本新构建的F2群体中的1 348个单株,共筛选出57株重组交换单株;在cp基因初始精细定位的220 kb区间内,利用黄瓜基因组测序结果及生物信息学分析,在标记区域内新开发了68对微卫星标记和1对序列标签位点(STS)标记,2对简单重复序列(SSR)标记和1对STS标记被成功用于交换单株的分析,并结合cp基因初始精细定位所用F2群体的1 273株单株的数据,最终利用2 621 F2单株将cp基因定位在178 kb的区间内,其共分离标记是SSR标记UW057998和STS标记cp-STS-6。最近的两侧翼标记CKX-indel和UW058058与cp基因分别相距0.04和0.23 cM,此结果排除了CKX作为cp候选基因的可能性,因此矮生基因的候选基因定位在标记CKX-indel和UW058058的178 kb区间,本研究结果不仅为进一步筛选和确认cp基因提供了基础数据,也为黄瓜矮生性状的分子标记辅助育种提供了科学依据。The compact plant architecture is an important agronomic traits in cucumber（Cucumis sativus L.） breeding, it has the potential to be used in high-density, efficient cultivation and once-over mechanical harvesting of cucumber production. Compact growth habit is controlled by a simply inherited recessive compact gene （cp）. Initial fine genetic mapping with 1 273 F2 plants has delimited the cp locus to a 220 kb genomic DNA region which is cosegregated with cytokinin oxidase gene（CKX）. To narrow down the region of cp gene, a new set of large F2 individuals was constructed, which was derived from the same two inbred cucumber lines, WI7201 （compact vining） and WI7200（regular vining）. Recombinant plants were screened by the flanking markers UW084680 and UW084870, which came from cp gene fine mapping in 2011, and among the 1 348 F2 plants, there were 57 recombinants between them. Based on the cucumber genetic and physical map information in this region, sixty-eight SSRs（simple sequence repeat） and one STS （sequence-tagged sites, STS） marker between UW084686 and UW084680 were designed and screened for polymorphism between the two parents. Two SSRs and one STS marker were used to further analysis. 2 621 F2 plants were used for fine genetic mapping ofcp which included 1 348 F2 plants from the new set ofF2 population and 1 273 F2 plants from cp gene fine mapping in 2011. Among the 2 621 F2 plants examined, no recombination was found between the compact locus and the two markers UW057998 and cp-STS-6. Finally the cp locus was delimited to a 178 kb genomic DNA region. Mapping effort thus far resulted in two flanking markers, CKX-indel and UW058058, which was 0.04 and 0.23 cM away from the cp locus, respectively. This result excludes the possibility of CKX as a candidate gene. As a result, the candidate gene ofcp is located in the 178 kb region between CKX-indel and UW058058. The results will lay the foundation not only for screening and confirmation cp gene but also for molecular marker-assisted bre

关 键 词：黄瓜 矮生基因(cp) 精细定位 微卫星标记 序列标签位点 

分 类 号：S722[农业科学—林木遗传育种]