利用AhMITE转座子分子标记研究花生栽培种及高世代材料的亲缘关系  

Genetic Relationship of Peanut(Arachis hypogaea L.) Varieties and Advanced Generation Lines Evaluated by AhMITE Transposable Markers

作　　者：王辉[1] 李双铃[1] 任艳[1] 许梦琦[1] 石延茂[1] 袁美[1] 王传堂[1] 

机构地区：[1]山东省花生研究所,农业部花生生物学与遗传育种重点实验室,山东省花生重点实验室,青岛266100

出　　处：《农业生物技术学报》2013年第10期1176-1184,共9页Journal of Agricultural Biotechnology

基　　金：山东省博士基金项目(No.BS2011NY014);国家科技支撑计划课题(No.2011BAD35B04);留学人员科技活动择优资助项目(No.2011)

摘　　要：花生微小倒转重复转座因子（AhMITE）标记是一种基于PCR的分子标记,其在花生中扩增的产物片段差异大,多态性强,带型简单、稳定,重复性好,采用琼脂糖凝胶电泳即可区分.本研究以26份花生栽培品种和89个高世代育种品系为研究材料,通过AhMITE分子标记技术分析其DNA多态性并进行聚类分析.研究共选用了多态性较好的33对AhTE引物,其中31对引物获得了较好的扩增效果,每对引物可扩增1～3条带,在115份材料共扩增产生57条带,其中多态性条带数为54,多态性条带比率为96.49％,115份材料间遗传相似系数平均值为0.6902.在遗传相似系数为0.65时可将所有材料分为3大组,来自同一省份的品种聚为一类,89个高世代品系中来自同一组合的品系优先聚为一类,用于分子标记辅助选育优良品种.本研究结果进一步证实,AhMITE分子标记作为一种新兴的分子标记,具有操作性强、多态性高、带型简单稳定等其它标记无可比拟的优点,可以广泛地用于花生遗传多样性研究、花生品种的鉴定以及花生遗传图谱的构建,并可用于分子标记辅助选育优良品种.Molecular marker technique has become the important tool for genetic diversity analysis. Miniature inverted-repeat transposable element（MITE） transposable marker is a type of PCR marker with high polymorphism and easy detection by agarose gel electrophoresis in peanut（Arachis hypogaea L.）. 33 pairs of AhTE primers were used to amplify 115 peanut accessions （including 26 varieties and 89 advanced generation lines）, 31 pairs got better results and each pair produced 1-3 bands with average bands of 1.84. Out of 57 bands amplified among 115 peanut accessions, 54 bands were polymorphic with polymorphic ratio of 96.49%. Average coefficient of genetic similarity（Gs） was 0.6902 ranged from 0.4035 to 0.9825. The maximal Gs was from peanut variety Mi-2 and Mi-3 and minimum was from lines F0316-1-1 and H0502-5-2-1. Cluster analysis was carried out using NTSYS-pc V2.10 software based on unweighted pair-group method with arithmetic means（UPGMA）. At the Gs of 0.65, all accessions were classified into 3 groups A, B and C. Group A consisted of 9 varieties and 11 lines, Group B was made up of 94 accessions including 16 varieties and 78 lines, and Group C had only one line NP13-2-1-3. Lines derived from same hybridization combination or varieties from same province were clustered into one group, which showed genetic relationship evaluated by MITE transposable markers consisted with their pedigree or origin. Results above suggested that AhMITE transposable marker is a useful marker for genetic diversity of peanut varieties and lines.The study also revealed that it is worth considering the use of AhMITE as DNA markers, AhMITE has many useful characteristics that other DNA markers do not have, it will contribute to the construction of linkage maps, evolutionary studies on the Arachis genome, and as a convenient tool for molecular breeding, it can assist in the progression of genetics, genomics and the breeding of peanut and its relatives.

关 键 词：花生 微小倒转重复转座因子(AhMITE) 分子标记 遗传多样性 

分 类 号：S562.03[农业科学—作物学]