斑茅野生种质资源SRAP遗传多样性研究  

Genetic Diversity of Native Erianthus arundinaceus Germplasm Detected by SRAP Markers

作　　者：张建波[1,2,3] 鄢家俊[2] 白史且[1,2] 张蕴薇[4] 刀志学[2] 李达旭[2] 张昌兵[2] 游明鸿[2] 张玉[2] 张劲[2] 杨富裕[4] 

机构地区：[1]四川农业大学草业科学系,雅安625014 [2]四川省草原科学研究院,成都611731 [3]贵州省草业研究所,贵阳550006 [4]中国农业大学草地研究所,北京100094

出　　处：《农业生物技术学报》2013年第10期1193-1202,共10页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(863)项目(No.2012AA101801-01);国家现代农业产业技术体系建设专项资金(No.CARS35-35);贵州省农业科学院研究生创新基金(No.2011014)

摘　　要：斑茅（Erianthus arundinaceus）是甘蔗育种的重要种质资源,同时也是潜在的能源草植物,对其进行遗传多样性研究是合理利用该资源的前提.本研究利用相关序列扩增多态性（SRAP）分子标记对我国7个省的45份野生斑茅种质资源进行遗传多样性研究,结果表明,1）20对引物组合共扩增出434条清晰的条带,多态性条带300条,占69％,材料间的遗传相似性系数（Gs）范围为0.703～0.986,平均Gs为0.842,说明我国野生斑茅资源具有丰富的遗传多样性;2）通过非加权配对类平均法（UPGMA）聚类分析和主成分分析发现,供试材料可以分为3个大类7个亚类,聚类结果呈现出较强的地域分布规律,材料间的遗传距离与地理距离有一定的相关性;3）我国野生斑茅资源的遗传多样性及聚类结果受地理条件的影响,大洋、山体能阻碍不同地域斑茅间的基因交流,而河流能促进基因交流.本研究揭示了我国野生斑茅的遗传多样性水平,阐明了影响其遗传多样性的主要因素,为斑茅的开发利用及甘蔗的遗传改良育种提供重要基础资料.Erianthus arundinaceus is not only an important sugarcane breeding germplasm resources, but also a potential energy grass plants, and the genetic diversity is the premise of utilization of E. arundinaceus. In this experiment, we analyzed genetic diversity of 45 accessions of E. arundinaceus collected from natural habitats in seven Chinese provinces using 20 sequence-related amplified polymorphism（SRAP） primer pairs （PPs）. Results showed that： 1） 20 PPs generated 434 bands and 69% was polymorphic bands. The Nei＇s genetic similarity（Gs） coefficient of the tested accessions ranged from 0.703 to 0.986, and the average Nei＇s Gs coefficient was 0.842. There was rich genetic diversity among the tested wild resources or E. arundinaceus. 2） The tested accessions were clustered into 3 groups and 7 subgroups by unweighted pair-group method with arithmetic means（UPGMA） dendrogram and principal component analysis. The cluster results demonstrated a strong geographic effect on molecular variation of the local E. arundinaceus, and there was no significant relationship between genetic distance and geographic distance among accessions. 3） The genetic diversity and cluster results were affected by geographic landforms and environments, the gene flow was blocked by Ocean and mountains, and was promoted by river. A high level of genetic variation of E. arundinaceus and main impact factors of genetic variation are presented, this conclusion will be highly valuable in genetic improvement in the species per se and likely in sugarcane.

关 键 词：斑茅 相关序列扩增多态性(SRAP) 遗传多样性 聚类分析 主成分分析 Mantel检验 

分 类 号：S668.4[农业科学—果树学]