去势对猪脂肪沉积及其关键基因表达的影响  

Effects of Castration on Fat Deposition and Its Related Gene Expression in Adipose Tissues of Male Pigs(Sus scrofa)

作　　者：蔡兆伟[1,2] 陈民利[1] 张立凡[2] 周红梅[2] 徐宁迎[2] 

机构地区：[1]浙江中医药大学动物实验研究中心/比较医学研究中心,杭州310053 [2]浙江大学动物科学学院,杭州310058

出　　处：《农业生物技术学报》2013年第10期1203-1209,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.31200921和No.31000997);浙江省自然科学基金项目(No.LQ12C04003)

摘　　要：去势和不去势公猪(Sus scrofa)在体脂沉积能力上存在显著差异,但导致这一现象的机理却不清楚。本研究采用荧光定量PCR技术分析了去势和不去势公猪不同部位脂肪组织中脂肪沉积关键基因的表达差异,并检测了去势对公猪体内脂肪沉积以及血清中相关激素水平的影响。结果显示,公猪去势后,血清中睾酮水平显著下降(P<0.01),但血清瘦素水平显著上升(P<0.01)。去势公猪体重(133.29±7.25)kg显著低于不去势公猪(120.60±4.77)kg(P<0.05),但胴体脂肪含量包括脂肪重,板油重以及肩部、背部背膘厚均显著高于不去势公猪(P<0.05)。另外,在皮下脂肪组织中,去势公猪脂肪合成相关基因过氧化物酶体增殖复合物激活受体γ(PPARγ),脂肪酸合成酶(FAS),乙酰辅酶A羧化酶(ACC),硬脂酰辅酶A去饱和酶(SCD)和苹果酸酶(ME1)基因的相对表达量均显著上调(P<0.05);而在腹部脂肪组织中,去势公猪腹部脂肪组织中只有FAS和ACC基因的相对表达量高于不去势公猪(P<0.05)。另外,固醇调节元件结合蛋白-1C(SREBP-1C)基因的相对表达量在两种脂肪组织中都没有显著变化(P>0.05)。本研究表明,公猪去势后具有更强的脂肪沉积能力,并且脂肪组织中脂肪合成基因表达高于不去势公猪。去势公猪体内增加的脂肪沉积可能和其脂肪组织中PPARγ,FAS,ACC,SCD和ME1基因的表达上调有关。The metabolism of adipose tissue is known to regulated by gonadal steroid hormones such as testosterone. Castrated and intact male pigs（Sus scrofa） demonstrate striking difference in body fatness, but up to now, the molecular mechanisms underlying such difference are still not clear. To determine whether the increased body fat mass in castrated male pigs is associated with the changes of gene expression in adipose tissues, we compared the mRNA expression levels of genes related to fat deposition in both subcutaneous and abdominal fat adipose tissue depots between intact and castrated male pigs using Real-time PCR method. Moreover, effects of castration on body fatness trait and serum hormones concentrations were also investigated in the present study. The results showed that castration could significantly reduce serum testosterone concentrations （P〈0.01）, while serum leptin concentrations were significantly increased by castration in male pigs （P〈0.01）. The castrated male pigs had lower body weight compared to the intact male pigs （120.6±4.77） kg vs （133.29±7.25） kg（P〈0.05）, but the levels of adiposity were higher in castrated male pigs than in intact male pigs. For example, the carcass fat weight, leaf fat weight, back fat thickness at shoulder and back fat thicknesses at buttock were all significantly higher in castrated male pigs than those in intact male pigs （P〈0.05）. Additionally, castration of male pigs caused a significant increase in peroxisome proliferator-activated receptor У（PPART）, fatty acid synthase（FAS）, acety-CoA carboxylase（ACC）, steraroyl- CoA desaturase（SCO） and malic enzyme 1（ME1） mRNA expression in subcutaneous fat adipose tissue （SAT） （P〈0.05）. The mRNA expression levels of sterol regulatory binding protein-1C （SREBP-1C） gene was not significant different in SAT adipose tissue between intact and castrated male pigs （P〉0.05）. The expression changes of lipogenic genes in castrated male pigs were different between a

关 键 词：猪 雄激素缺乏 脂肪 肥胖 

分 类 号：S828[农业科学—畜牧学]