纳米羟基磷灰石/壳聚糖(n-HA/CS)复合材料对SD大鼠代谢器官的影响  

Effects of Nano-Hydroxyapatite /Chitosan( N-HA /CS) on MC3T3-E1 Cell and Metabolic Organ in SD Rats

作　　者：王丽婷[1,2] 周钢[1] 樊瑜波[1] 

机构地区：[1]北京航空航天大学生物与医学工程学院,北京100191 [2]国家康复辅具研究中心,北京100176

出　　处：《中国生物医学工程学报》2013年第5期595-600,共6页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金重大国际合作研究项目(11120101001);国家自然科学基金(10925208)

摘　　要：纳米羟基磷灰石/壳聚糖复合材料(n-HA/CS)是目前骨修复替代领域使用广泛的一种生物材料。为探讨该材料在体内经代谢降解后,对大鼠体内代谢器官生理功能的影响,并分析其可能的作用机制。本研究用共沉淀法制备n-HA/CS复合材料,并采用红外光谱分析(IR)、X射线衍射(XRD)等手段表征该材料。通过细胞生长抑制实验,病理切片以及血清生化指标的检测,从体内体外两种方式观察该材料对MC3T3-E1细胞以及SD大鼠肝脏、肾脏等代谢器官的影响。结果显示,100 mg/kg含量n-HA/CS复合材料粉末在大鼠体内代谢8周,血清尿素氮(BUN)水平升高为29.6 mmol/L;肌酐(CR)水平升高为66.9 umol/L;总胆红素(T-B)及间接胆红素(I-B)水平也分别升高至1.79 umol/L和1.32 umol/L,均较对照组水平明显增高,有显著性差异(P≤0.05)。且肝脏、肾脏组织中出现大量凋亡细胞,无组织坏死和炎症表现。n-HA/CS复合材料在体内代谢8周后,通过诱导细胞凋亡影响机体肝、肾等代谢器官的功能。Composite conmposed of nano-hydroxyapatite and chitosan （n-HA/CS） is widely used in the field of bone repair and alternative. To investigate the degradation effects of n-HA/CS on metabolism organs in SD rat and analyze the possible mechanism, particulate n-HA/CS was prepared by precipitation and characterized by IR and XRD. Serum biochemical parameters measurement and histopathologieal analysis were performed to evaluate the composite toxicity after 8 weeks of composite implantation （100 mg/kg）. Results show that blood urea nitrogen （BUN） levels was 29.6 retool/L, creatinine （CR） levels was 66.9 umol/L, total bilirubin （T- B） and indirect bilirubin （I-B） levels were also increased to 1.79 umol/L and 1.32umol/L. Compared with that of control, there were significant differences （P~〈0.05）. A large number of apoptotic instead of necrosis cells were observed in the liver and renal proximal tubule tissue. It is concluded that the toxicity of n-HA/CS is attributable to the cells apoptosis.

关 键 词：纳米羟基磷灰石 壳聚糖复合材料 凋亡 代谢 

分 类 号：R318[医药卫生—生物医学工程]