内皮祖细胞条件培养基对VSMCs增殖、迁移及粘附功能的影响  

Effects of endothelial progenitor cell-conditioned medium on the proliferation,adhesion and migration of vascular smooth muscle cells

作　　者：吴海燕[1] 楚海荣[2] 李宏[2] 唐可欣[1] 靖旭[1] 孙金隆[2] 尹青令[2] 成敏[2] 

机构地区：[1]潍坊医学院麻醉学系 [2]潍坊医学院医学研究实验中心,山东潍坊261053

出　　处：《中国应用生理学杂志》2013年第3期199-202,I0002,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(30900290;31270993);教育部新世纪优秀人才支持计划资助(NCET-10-0922);山东省高等学校科技计划项目(J11LF17);潍坊市科技发展计划(20121228)

摘　　要：目的：探讨大鼠骨髓来源的内皮祖细胞条件培养基（EPC-CM）对大鼠血管平滑肌细胞（VSMCs）增殖、迁移及粘附功能的影响。方法：密度梯度法获取大鼠骨髓单核细胞,体外培养EPCs,进行鉴定,并制备EPC-CM。同时采用组织块贴壁法获取VSMCs,以传至第3~5代的VSMCs为靶细胞。经EPC-CM处理24 h后,采用CCK-8、粘附能力测定实验及改良Boyden小室检测EPC-CM对VSMCs增殖、迁移及粘附的影响。结果：与对照培养基比较,制备的EPC-CM明显抑制了VSMCs的增殖、粘附及迁移能力（P〈0.01）。结论：EPC-CM有效抑制了VSMCs的增殖、迁移以及粘附能力,这可能是EPCs抗动脉粥样硬化等心血管疾病发生的原因之一。Objective： To investigate the effects of endothelia progenitor cclls conditioned medium（EPC-CM） on the migration,adhesion and proliferation of vascular smooth muscle cells（VSMCs）.Methods： Mononuclear cells were isolated from rat bone marrow by density gradient centrifugation,plated on dishes precoated with 5% fibronectin,and then cultured with complete M199 medium（including 15% fetal calf serum,10 μg/L VEGF and 5 μg/L bFGF）.EPC-CM was collected and used to incubate VSMCs isolated from rat arteriae aorta.After 24 h,VSMCs proliferation,adhesion and migration were assayed with CCK-8,adhesion test and modified Boyden chamber assay,respectively.Results： The proliferation,adhesion and migration of VSMCs were obviously decreased when the cells were cultured with EPC-CM.Conclusion： EPC-CM could inhibit VSMC functions,which would be one of the mechanisms against atherosclerosis by EPCs.

关 键 词：动脉粥样硬化 内皮祖细胞 血管平滑肌细胞 

分 类 号：R331.3[医药卫生—人体生理学]