低温弱光下类囊体腔内质子由CF_(0)渗漏引起黄瓜类囊体耦联度降低  

H^(+)leakage through CF_(0) decreases the coupling degree of thylakoids from cucumber leaves exposed to low light at low temperature

作　　者：陈启林[1] 山仑[2] 程智慧[1] 沈允钢[3] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100 [2]中国科学院水土保持研究所,陕西杨陵712100 [3]中国科学院上海植物生理生态研究所,上海200032

出　　处：《西北植物学报》2000年第6期984-991,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家重点基础研究发展规划项目资助(G1999011708)

摘　　要：毫秒延迟发光测定结果表明低温弱光处理黄瓜叶片导致类囊体原位 (in situ)耦联度显著降低。DCCD可以恢复低温弱光处理的黄瓜叶片的毫秒延迟发光的慢相强度和反映类囊体膜质子吸收的 9- AA(9- Aminoacridine)荧光猝灭能力 ,说明类囊体耦联度降低的原因是质子由 CF0 大量快速渗漏。进一步研究结果表明 ,活性氧和 CF1的脱落不是低温弱光引起黄瓜类囊体耦联度降低的根本原因。Results from m DLE measurement of cucumber leaves indicated that \%in situ\% thylakoid coupling degree of cucumber were decreased by low light at low temperature.DCCD could restore not only the slow phase intensity of ms DLE of cucumber leaves but the ability to eliminate 9 AA fluorescence,which meant that the decrease of thylakoid coupling degree resulted from fast H\++ leakage through CF 0.Further studies suggested that reactive oxygen species and CF 1 dissociation from ATPase were not direct cause of the decrease in question.

关 键 词：低温弱光 黄瓜 解耦联 毫秒延迟发光 9-AA荧光 

分 类 号：S642.201[农业科学—蔬菜学]