戊巴比妥钠处理后SD大鼠体内外精子运动功能分析  

Analysis of sperm motility in rats after pentobarbital sodium treatment in vivo and in vitro

作　　者：宋翼升[1] 周国亮[1] 由振强[1] 辛艳飞[1] 黄敏聪[1] 宣尧仙[1] 

机构地区：[1]浙江省医学科学院安全性评价研究中心,浙江杭州310013

出　　处：《中国药理学与毒理学杂志》2013年第5期843-847,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：浙江省自然科学基金(LY12H31008);浙江省医药卫生平台骨干人才计划(2012RCB008);浙江省科技厅项目(2010F10026);国家"十二五"科技重大专项(2011ZX09301-003)~~

摘　　要：目的观察戊巴比妥钠对大鼠体内外精子运动功能的影响。方法①体外实验：大鼠精子悬液密度为（6．36—6．78）×10^5^L-1，加入戊巴比妥钠使其终浓度为O，0。1，1，10，100和1000umol·L^-1，孵育1和2h后，分别用计算机辅助精子分析仪检测精子活力及运动功能参数，包括曲线运动速度（VCL），平均路径速度（VAP），直线运动速度（VSL），鞭打频率（BCF），精子头侧摆幅度（ALH），直线性（LIN）和前向性（STR）。②体内实验：大鼠ip给予戊巴比妥钠0．04g·kg^-1，于15，60及120min后迅速取出单侧附睾尾，制备精子悬液；精子悬液稀释密度为（4．95—7．46）×10^5L^-1，用计算机辅助精子分析仪检测精子活力及运动功能参数VCL，VAP，VSL，BCF，ALH，LIN和STR。结果①体外实验：与正常对照组相比，戊巴比妥钠0．1，1，10，100和1000umol·L^-1对精子活力及运动参数VAP，VSL，VCL，ALH，BCF，STR和LIN无明显影响，单个精子运动轨迹呈直线前进性，与正常对照组相似。②体内实验：大鼠经ip给予戊巴比妥钠后，精子运动功能参数VAP，VSL，VCL，ALH，BCF，LIN和STR与正常对照组比较均无显著性差异，单个精子运动轨迹呈直线前进性，与正常对照组相似。结论在本实验条件下，戊巴比妥钠对精子的运动功能无明显影响，可以作为生殖毒性实验雄性大鼠的麻醉用药。OBJECTIVE To evaluate the effect of pentobarbital sodium on sperm motility （MOT） in rats. METHODS ① In vitro Sperms from distal cauda epididymis in rats were suspended in M199 supplemented with 0.5% fetal calf serum and sperm concentration was adjusted to about （6.36-6.78） ×10^5L^-1. The sperm suspensions were incubated with pentobarbital sodium 0, 0.1, 1, 10, 100, 1000 umol·L^-1 for 1 and 2 h. MOT was assessed by computer-aided sperm analysis. ② In vivo Rats was ip given pentobarbital sodium 0.04 g·kg^-1. After 15, 60, 120 min pentobarbital sodium exposure, sperms were collected from the distal cauda peididymis and sperm suspension （4.95-7.46） ×10^5L^-1 was prepared. The sperm curvilinear velocity （VCL）, average path velocity （VAP）, straight line velocity （VSL）, beat cross frequency （BCF）, amplitude of lateral head movement （ALH）, linearity（LIN）, and straightness（STR） in the cauda epididymis in vitro and in vivo were assessed by computer-aided sperm analysis. RESULTS ① In vitro Compared with normal control group, there were no significant differences in motion parameters MOT, VCL, VAP, VSL, BCF, ALH, LIN and STR in pentobarbital sodium groups. The sperm in the pentobarbital sodium groups exhibited progressive and straight courses, as in the control group. ② In vivo There was no obvious difference between pentobarbital sodium groups and normal control group. The sperm exhibited progressive and straight courses as in the normal control group. CONCLUSION Pentobarbital sodium does not affect sperm motion as assessed, which is potentially applicable to reproductive toxicology studies.

关 键 词：戊巴比妥钠 大鼠精子 精子运动功能 

分 类 号：R965[医药卫生—药理学]