实时xCELLigence细胞分析系统在药物心脏毒性筛选中的应用  

Progress in real time xCELLigence analysis system on drug cardiotoxicity screening

作　　者：王淑颜 汪溪洁[1,2,3] 马璟[1,2,3] 

机构地区：[1]中国医药工业研究院 [2]上海医药工业研究院 [3]国家上海新药安全评价研究中心,上海201203

出　　处：《中国药理学与毒理学杂志》2013年第5期908-912,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家科技重大专项(2012ZX09302002)~~

摘　　要：药物的心脏毒性越来越受到关注,大量临床上使用的药物由于心脏毒性而被撤市或监管。因此,药物安全评价是研发过程中非常重要的一步。实时xCELLigence细胞分析系统是一种基于电子阻抗的非标记性、非侵入性、实时动态的细胞分析检测技术,它可以广泛地应用于药物的毒性筛选中。xCELLigence RTCA Cardio系统具有高的时间分辨率,能灵敏地、精确地监测到药物作用于心肌细胞后心肌细胞搏动频率、节律、幅度等指标的改变,并通过Poincaréplots分析其心率变异性。这些指标均与药物的心脏毒性有着密切的关系。因此,该系统具有高的特异性,能在药物研发的早期阶段筛选其心脏毒性。本文对RTCA的基本原理和其在药物心脏毒性筛选中的应用作一综述。Cardiotoxicity of drugs are attracting more public attention, as a large number of clinical drugs have been withdrawn from the market or safety labels have been revised due to their cardiotoxicity. Therefore, safety assessment remains a crucial step for drug development. Real time xCELLigence analysis system is an impedance-based cell detection platform that provides a non-invasive, label-free way to continuously cellular monitor and can be used widely in the drug cardiotoxicity screening. On one hand, with the high time resolution, xCELLigence RTCA Cardio system cellular monitors the drug effect sensitively and accurately, such as beating rate, rhythm, amplitude. On the other hand, Poincar~ plots can analyze the heart rate variability. All these indexes are closely related to drug cardiotoxicity, making this system highly specific to screeing potential drugs for cardiotoxic effects.

关 键 词：实时细胞分析 xCELLigence 心脏毒性 早期筛选 安全性评价 

分 类 号：R96[医药卫生—药理学]