耐铬沙雷氏菌CQMUS2 FMN_red基因片段的克隆与序列分析  

Cloning and Sequence Analysis of FMN_red Gene Fragment from Serratia sp. CQMUS2

作　　者：谭小庆[1] 白群华[1] 韩令力[1] 曹显庆[1] 肖虹[1] 

机构地区：[1]重庆医科大学公共卫生与管理学院,重庆400016

出　　处：《生物技术》2013年第5期8-11,共4页Biotechnology

基　　金：重庆市自然科学基金项目(2010BB5360)资助

摘　　要：目的:克隆沙雷氏菌CQMUS2核黄素单核苷酸还原酶(FMN reductase)。方法:用水煮模板法提取沙雷氏菌CQMUS2基因组DNA,根据已知沙雷氏菌AS13 FMN还原酶基因的DNA同源区域,设计1对特异性引物,PCR扩增出沙雷氏菌CQMUS2FMN还原酶基因片段,采用生物信息学软件进行分析。结果:获得一段大小为305 bp的基因片段,开放阅读框为303 bp,编码101个氨基酸残基;该基因核苷酸序列与其他细菌的FMN还原酶基因的同源性均在83%以上,氨基酸序列同源性达89%以上。结论:成功地克隆了沙雷氏菌CQMUS2 FMN还原酶DNA的部分序列,为以后获取FMN还原酶DNA的全长序列及其生物学功能研究提供基础。Objective： To clone FMN reductase gene from Serratia sp. CQMUS2. Method： Genomic DNA from Serratia sp. CQMUS2 was extracted by boiled template method. A pair of specific primers was designed based on the homologous sequence of FMN reductase gene from Serratia sp. ASI 3, the FMN reductase gene fragment was amplificated by PCR, and then sequenced and analyzed. Result：The FMN reductase gene fragment was 305 bp long and contained an open reading frame （ORF） of 303 bp that encoded 101 amino acids. Comparing the nucleotide sequence and amino acid sequence of the FMN reductase gene with other bacteria showed that it is above 83% and 89% . Conclusion： The FMN reductase gene fragment was successfully cloned, which laid a foundation for further obtaining its full-length gene and studying biological functions.

关 键 词：沙雷氏菌CQMUS2 FMN还原酶基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]