苦白蹄子实体总三萜的提取工艺研究  

Optimization of Extraction of Total Triterpenoids from Fomes officinalis (Vill.:Fr.) Bond

作　　者：许培源[1] 袁博[1] 李貌[2] 宋玲珊[2] 蒋继宏[1] 

机构地区：[1]江苏省药用植物生物技术重点实验室,江苏师范大学,江苏徐州221116 [2]江苏师范大学科文学院,江苏徐州221116

出　　处：《生物技术》2013年第5期90-95,共6页Biotechnology

基　　金：国家自然基金项目("杨树内生放线菌抗杨树黑斑病菌活性成分及其作用机理研究";31170605);江苏高校优秀科技创新团队资助项目(药用微生物前期开发);江苏师范大学校自然科研基金项目("药食真菌来源的壳聚糖对紫甘薯花青素吸附机理及活性研究";12XLB04)资助

摘　　要：目的:探索苦白蹄子实体总三萜最佳提取条件。方法:通过利用超声提取、热提取、微波提取三种方法对苦白蹄子实体进行提取,并对总三萜含量进行检测,从而优选了超声提取法。在对超声提取时间、超声设定功率、超声温度和液料设定比例单因素试验的基础之上,根据中心组合设计原理采用四因素三水平的响应曲面分析法,通过建立数学模型对各因素显著性和交互性的作用分析。结果:得到了超声提取苦白蹄子实体总三萜的最佳工艺条件为提取溶剂为80%乙醇,超声提取时间为50min,提取温度为60℃,液料设定比例为45:1(mL/g),超声设定功率为450W。结论:在这此条件下总三萜的实际提取率为26.44%。Objective： To optimize extraction conditions of total triterpenoids of Fomes offwinalis （ Vill. ： Fr. ） Bond. Method： In this exper-iment, ultrasonic - aided extraction, heating extraction and microwave - assisted extraction were carried out to detect the content of total trit-erpenoids of bbmes officinalis （ Vill. ： Fr. ） Bond. And ultrasonic - aided extraction was chosen as the best extraction method. On the basic of a series of single factor experiments of ultrasonic treatment time, temperature, material/liquid ratio and ultrasonic power was carried out. The four - variable and three - level response surface methodology based on the principle of central composite design was employed to analyze the significance and interactions of the above four factors affecting the yield of total triterpenoids by mathematical modeling. Result：The optimal conditions were determined as follows： ultrasonic treatment time, 50 rain, extraction solvent, 80% ethanol, extraction temperature,60℃ ,material/liquid ratio,45：1, and ultrasonic power,450 W. Conclusion：The actual yield of total triterpenoids was 26.44% under such conditions.

关 键 词：苦白蹄 相应曲面 超声波提取 总三萜 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]