玻璃化冻存技术对大豆根瘤菌生物学活性的影响  

Effect of Vitrification Preservation Technology on Biological Activity of Rhizobium

作　　者：于德水[1,2] 赵晓宇[1,2] 奚新伟[1,2] 戴肖东[1,2] 张先成[2] 王天亮[3] 姜宏宇[1,2] 沙长青[3] 

机构地区：[1]黑龙江省科学院高技术研究院,黑龙江哈尔滨150020 [2]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [3]黑龙江省科学院,黑龙江哈尔滨150001

出　　处：《生物技术》2013年第5期95-98,共4页Biotechnology

基　　金：黑龙江省财政基本科研业务费专项项目("大豆根瘤菌菌种保藏中玻璃化技术操作规范的建立";编号:CZ12BYDS06);黑龙江省科技攻关项目("黑龙江省微生物肥料产业发展模式研究;编号:GC12D303)资助

摘　　要：目的:研究探讨玻璃化技术在根瘤菌保藏中的应用,并开展相关实验。方法:分别选取快生根瘤菌、慢生根瘤菌各1株为初始菌株,以30%甘油:15%乙二醇:15%DMSO为玻璃化冻存试剂,对大豆根瘤菌进行玻璃化冻存,分别选取冻存1个月、6个月以及12个月的菌株进行存活率以及固氮酶活性测定,研究玻璃化技术对根瘤菌生物学功能的影响。结果:保藏12个月后,大豆快生根瘤菌、慢生根瘤菌菌株存活率均可达到85%以上,固氮酶活性没有明显下降。结论:所用玻璃化方法及试剂对快生大豆根瘤菌保藏具有很好的作用,对慢生根瘤菌的保藏效果还需进一步实验验证。Objective：The possibility of vitrification technology applied in Rhizobium preservation was studied, and some related experi-ments were done. Method：Sinorhizobiurn fredii and Bradyrhizobium were used as the initial strains, respectively. Vitrification agent was made from 30% glycerol, 15% ethylene glycol and 15% DMSO. Two strains were stored in the vitrification agent for 1 month ,6 months and 12 months, respectively. Then the survival rate and nitrogenase activity of the strains were determined. Result： The survival rate of the strains were more than 85% and the nitrogenase activity did not decrease remarkably after 12 months. Conclusion：There was good effect on Sinorhizobium fredii preservation with the vitrification method and reagent used in this paper, but it needs further study for Bradyrhizobi-um.

关 键 词：根瘤菌 玻璃化技术 存活率 固氮酶活性 

分 类 号：Q93-336[生物学—微生物学]