糖耐康对TGF-β_1诱导的HK-2细胞VEGF mRNA表达的影响  

Effects of TangNaiKang on VEGF mRNA Expressions of Human Renal Tubular Epithelial Cells HK-2 Induced by TGF-β_1

作　　者：朱春晖[1] 康开彪[2] 杨丽霞[2] 李晓东[2] 刘铜华[3] 吴丽丽[3] 孙文[3] 

机构地区：[1]甘肃省人民医院,甘肃兰州730000 [2]甘肃省中医药研究院 [3]北京中医药大学

出　　处：《西部中医药》2013年第9期13-15,共3页Western Journal of Traditional Chinese Medicine

基　　金：国家自然基金面上项目(编号30973909);北京中医药大学创新团队项目(编号2011-CXTD-19)

摘　　要：目的:通过观察糖耐康药物血清干预转化生长因子-β1(TGF-β1)诱导的人肾小管上皮细胞(HK-2)、血管内皮生长因子(VEGF)mRNA的表达,进一步探讨其在防治肾间质纤维化方面的作用。方法:将HK-2细胞用含10%胎牛血清的DMEM/F12(1∶1)培养基培养;实验分为6组:空白对照组、单纯TGF-β1诱导组(TGF-β110 ng/mL)、空白血清对照组(TGF-β110 ng/mL+10%空白血清)、干预1组(TGF-β110 ng/mL+5%糖耐康药物血清)、干预2组(TGF-β110 ng/mL+10%糖耐康药物血清)、干预3组(TGF-β110 ng/mL+20%糖耐康药物血清)。药物干预24小时后,荧光定量PCR检测VEGF mRNA的表达。结果:HK-2细胞经TGF-β1诱导后,VEGF mRNA的表达显著上升,与空白对照组相比有统计学意义(P<0.05),经糖耐康药物血清干预后,VEGF mRNA的表达逐步下降,与单纯TGF-β1诱导组相比有统计学意义(P<0.05)。结论:糖耐康能够抑制TGF-β1诱导的人肾小管上皮细胞VEGF mRNA的表达,在一定程度上具有抑制肾小管上皮细胞纤维化的作用。Objective：To explore the mechanism of TangNaiKang in preventing and treating renal interstitial fibrosis through observing the intervention of TangNaiKang on vascular endothelial growth factor （VEGF） mRNA expressions of human renal tubular epithelial cells（HK-2） induced by transforming growth factor （TGF-β1）. Method：HK-2 cells were cultured in the media of DMEM/F12 （1：1） containing 10%fetal bovine serum;the cells were divided into six groups： blank control group, TGF-β1 group （TGF-β110 ng/mL）, blank serum control group （TGF-β1 10 ng/mL＋10% blank serum）, intervention group one （TGF-β110 ng/mL＋5% medicated serum of TangNaiKang）, intervention group two （TGF-β1 10 ng/mL＋10% medicated serum of TangNaiKang） and intervention group three （TGF-β110 ng/mL＋20%medicated serum of TangNaiKang）. After intervened for 24 hours,VEGF mRNA expressions were detected with fluorescence quantitative PCR. Result：VEGF mRNA expressions were raised significantly when HK-2 cells were induced by TGF-β1, it had statistical meaning compared with blank control group （P〈0.05）, VEGF mRNA expressions w ere decreased gradually after intervened with medicated serum of TangNaiKang, it had statistical meaning compared with TGF-β1 group （P〈0.05）. Conclusion：TangNaiKang could inhibit VEGF mRNA expressions of HK-2 induced by TGF-β1 and the fibrosis of renal tubular epithelial cells to a certain extent.

关 键 词：糖耐康 转化生长因子-Β1 人肾小管上皮细胞(HK-2) VEGF mRNA 

分 类 号：R285.5[医药卫生—中药学]