止消通脉宁对TGF-β_1诱导的HK-2细胞Smad2、3、7 mRNA表达的影响  

The Effects of ZhiXiao TongMaiNing on Smad 2, Smad 3 and Smad 7 mRNA Expressions of Human Renal Tubular Epithelial Cells HK-2 Induced by TGF-β_1

作　　者：杨频 杨丽霞[2] 刘铜华[3] 吴丽丽[3] Margetts Peter Joseph 

机构地区：[1]甘肃省中医学校,甘肃兰州730050 [2]甘肃省中医药研究院 [3]北京中医药大学 [4]McMaster University Hamilton Ontario

出　　处：《西部中医药》2013年第9期16-19,共4页Western Journal of Traditional Chinese Medicine

基　　金：科技部国际科技合作项目(编号2009DFA31520);北京中医药大学创新团队项目(编号2011-CXTD-19)

摘　　要：目的:探讨止消通脉宁对转化生长因子-β1(TGF-β1)诱导的人肾小管上皮细胞(HK-2)Smad 2、Smad 3、Smad 7的影响。方法:将HK-2细胞用含10%胎牛血清的DMEM/F12(1:1)培养基培养;实验分为6组:空白对照组、单纯TGF-β1诱导组(TGF-β110 ng/mL)、空白血清对照组(TGF-β110 ng/mL+10%空白血清)、中药含药血清低剂量组(TGF-β110 ng/mL+10%低剂量止消通脉宁含药血清)、中药含药血清中剂量组(TGF-β110 ng/mL+10%中剂量止消通脉宁含药血清)、中药含药血清高剂量组(TGF-β110 ng/mL+10%高剂量止消通脉宁含药血清)。药物干预24小时后,荧光定量PCR检测Smad 2、smad 3、Smad 7的mRNA表达。结果:HK-2细胞经TGF-β1诱导后,Smad 2、smad 3的mRNA表达显著上升,Smad 7的mRNA表达下降,与空白对照组相比有显著性差异(P<0.05),但经止消通脉宁含药血清干预后,Smad 2、smad 3的mRNA表达逐步下降,Smad 7的mRNA表达上调,与单纯TGF-β1诱导组相比有显著性差异(P<0.05)。而空白血清无此作用。结论:止消通脉宁能够调控TGF-β1诱导的人肾小管上皮细胞转分化Smad信号通路mRNA表达的作用,在一定程度上具有抑制肾间质纤维化的作用。Objective：To explore the effects of ZhiXiao TongMaiNing on Smad 2, Smad 3 and Smad 7 of hu-man renal tubular epithelial cells （HK-2） which were induced by transforming growth factor （TGF-β1）. Method：HK-2 cells were cultured in the media of DMEM/F12 （1：1） containing 10%fetal bovine serum;the cells were divid-ed into six groups：blank control group, TGF-β1 group （TGF-β110 ng/mL）, blank serum control group （TGF-β1 10 ng/mL＋10%blank serum）, low dose group of TCM medicated serum （TGF-β110 ng/mL＋10%low dose medicated serum of ZhiXiao TongMaiNing）, moderate dose group of TCM medicated serum （TGF-β1 10 ng/mL＋10%moderate dose medicated serum of ZhiXiao TongMaiNing） and high dose group of TCM medicated serum （TGF-β1 10 ng/mL＋10%high dose medicated serum of ZhiXiao TongMaiNing）. After intervened for 24 hours, Smad 2, Smad 3 and Smad 7 mRNA expressions were detected with fluorescence quantitative PCR. Result：Smad2 and Smad3 mR-NA expressions were raised significantly, Smad 7 mRNA expressions were decreased when HK-2 cells were in-duced with TGF-β1, it had significant difference compared with blank control group （P〈0.05）, but after intervened with medicated serum of ZhiXiao TongMaiNing, Smad 2 and Smad 3 mRNA expressions were lowered gradually, Smad 7 mRNA expressions were improved, it had statistical meaning compared with TGF-β1 group （P〈0.05）. Blank serum presented no effects. Conclusion：ZhiXiao TongMaiNing could regulate mRNA expressions of trans-differenti-ated Smad signal channel of HK-2 cells induced with TGF-β1 and inhibit the fibrosis of renal tubular epithelial cells to a certain extent.

关 键 词：止消通脉宁 转化生长因子-Β1 HK-2细胞 Smad2mRNA Smad3mRNA SMAD 7mRNA 

分 类 号：R285.5[医药卫生—中药学]