有毒甲藻生物量快速测定的比较研究  

Comparative study on cell counting method with toxic dinoflagellate Amphidinium klebsii

作　　者：曾玲[1,2] 文菁[1] 龙超[2] 林伟源[1] 

机构地区：[1]湛江师范学院,广东湛江524048 [2]中国科学院南海海洋研究所/中国科学院海洋生物资源可持续利用重点实验室/广东省海洋药物重点实验室,广东广州510301

出　　处：《广东农业科学》2013年第20期151-154,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31201999);中科院重要方向性项目(KSCX2-YW-R-093);广东省自然科学基金(S2011040000463);广东高校优秀青年创新人才培养计划项目(LYM11086);国家星火计划项目(2012GA780007;2012GA780008);湛江市科技攻关计划项目(2012C3102018);湛江师范学院自然科学研究项目(QL1008)

摘　　要：采用显微镜计数法、荧光分光光度法、叶绿素a含量测定法、可见分光光度法对克氏前沟藻(Amphidinium klebsii)指数生长期不同浓度的培养液进行细胞密度测定。结果表明后3种细胞计数方法与显微镜计数法相比较,均有较好的线性关系;同时对不同生长期的克氏前沟藻培养液进行测定的结果表明,分光光度法的生长曲线和生长速率均与显微镜计数法有很好的一致性,两者之间有较好的线性关系。荧光法不能正确反映藻的生长态势,叶绿素a含量在稳定期出现下降,不能准确反映藻密度,这两种方法不适用于长期检测藻密度。通过比较分析发现,可见分光光度法适用于不同细胞密度范围的测定,操作简单、耗时短、不需要破坏样品、适于批量操作,可确定为便捷、有效、可行的细胞计数法。In this study four cell-counting methods of microscope, fluorescence spectrophotography, visible spectrophotography and Chlorophyll a containing measurement were used to measure the amount of the exponential phase cell of Amphidinium klebsii. The insightful comparison on the interrelations of the last three mentioned cell counting methods was made with the method of microscope, the results revealed the sound linear interrelations among them. Different growth phase of Amphidinium klebsii were simultaneously measured, and then growth curves were drew basis on the data from different methods. The results showed good concordance between microscope and visible spectrophotography methods, which revealed the sound linear interrelations. Because of the fluorescence spectrophotography method can not correctly reflect the algae growth, and the Chlorophyll a containing measurement can＇t accurately reflect the algae density which Chlorophyll a content declined in the stationary phase, both methods can＇t apply to detection the algae density for long-term. It found that the visible spectrophotography methods which operating procedures are simple, fast, suitable for wide cell-density ranges, batch measurements and the test sample cells are not destructed and can be reused for further research after measurement through comparative and analysis. Therefore, visible spectrophotography is convenient, high-efficiency and reliable approach for cell counting.

关 键 词：克氏前沟藻 细胞计数法 分光光度法 

分 类 号：S968.4[农业科学—水产养殖]