Comparison of Hematoxylin-eosin Staining and Methyl Violet Staining for Displaying Ghost Cells  

作　　者：Wenxin Zhang Yongping Li Jianxian Lin Shangtao Wan Huanhuan Gao Lanzhi Zhang Jianliang Zheng Ping Zhang 

机构地区：[1]State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University

出　　处：《Eye Science》2013年第3期140-143,共4页眼科学报（英文版）

摘　　要：Purpose:.To compare the merits and limitations of hematoxylin-eosin(HE) and methyl violet staining for displaying ghost cells from vitreous or aqueous humor.Methods:.A specimen containing ghost cells was adjusted to five different concentrations:(12×104,.10×104,.8×104, 6×104and 4×104cells / ml) and subjected to smearing and methyl violet and HE staining..The staining results were observed by light microscopy.Results: The ghost cells were readily observed at a cell density of > 8×104cells / ml with methyl violet staining,.but only a few cells were occasionally seen at lower cell densities..In contrast,.ghost cells were seen at all cell densities with HE staining.Conclusion: Methyl violet staining is more rapid and simpler for the identification of ghost cells, but its staining color more readily fades, the slides cannot be stored, and it is only effective at a cell density of > 8 ×104cells / ml. In contrast,.HE staining is more time-consuming but it can display cell morphology and distinguish cell components more explicitly and slides can be permanently stored. HE staining has advantages over methyl violet staining in detecting the ghost cells when the concentration is < 8×104cells / ml.Purpose：To compare the merits and limitations of hematoxylin-eosin （HE） and methyl violet staining for displaying ghost cells from vitreous or aqueous humor.Methods：A specimen containing ghost cells was adjusted to five different concentrations：（12×104,10×104,8×104,6×104 and 4×104 cells/ml） and subjected to smearing and methyl violet and HE staining.The staining results were observed by light microscopy.Results：The ghost cells were readily observed at a cell density of ＞ 8×104 cells/ml with methyl violet staining,but only a few cells were occasionally seen at lower cell densities.In contrast,ghost cells were seen at all cell densities with HE staining.Conclusion：Methyl violet staining is more rapid and simpler for the identification of ghost cells,but its staining color more readily fades,the slides cannot be stored,and it is only effective at a cell density of ＞ 8 ×104 cells/ml.In contrast,HE staining is more time-consuming but it can display cell morphology and distinguish cell components more explicitly and slides can be permanently stored.HE staining has advantages over methyl violet staining in detecting the ghost cells when the concentration is ＜ 8×104 cells/ml.

关 键 词：细胞密度 HE染色 甲基紫 苏木精 显示 伊红 光镜观察 电池组件 

分 类 号：Q2-33[生物学—细胞生物学]