废水脱氮与沼气脱硫耦联污泥微生物优势种群分析  

The Analysis of Microbial Ecological Communities in Sulfide and Nitrite Treatment System

作　　者：魏本平[1] 陈子爱[1] 丁晨[1] 苏小红[1] 卢秀红 邓良伟[1] 

机构地区：[1]农业部沼气科学研究所,成都610041 [2]吉林省临江市农村经营管理总站,吉林临江134600

出　　处：《中国沼气》2013年第5期10-14,28,共6页China Biogas

基　　金：国家自然科学基金项目(31101749);863项目(2013AA102805-02)

摘　　要：文章对于不同底物(含氮含硫猪场废水、含氮猪场废水通沼气)、不同接种污泥(厌氧污泥、好氧污泥)和不同微生物培养方式(悬浮培养、附着培养)驯化获得的同步脱氮除硫污泥,应用变性梯度凝胶电泳(DGGE)法比较不同驯化条件下同步脱氮脱硫耦联系统微生物种群差异,取得了较好的分离效果,显示了4种驯化方式脱氮脱硫耦联污泥系统中的微生物多样性。经鉴定得知优势种群为埃希氏-志贺菌属(Escherichia-Shigella)。Microbial population differences of simultaneous denitrification and desulfuration sludge obtained under different acclimation conditions as different substrates （ pig farm wastes containing both nitrogen and sulfur, nitrogenous pig wastes treated with biogas）, different inoculation sludge （anaerobic sludge, aerobic sludge） and different way of culturing （sus- pension culture, adherent cultivation）, were analyzed through DGGE method. The method obtained a good separation effect, and showed the microbial＇diversity in denitrifieation and desulfuration coupling system obtained from different accli- mation conditions. And Escherichia-Shigella was identified as dominant species.

关 键 词：猪场废水 脱氮 沼气 脱硫 DGGE 

分 类 号：X703[环境科学与工程—环境工程] S216.4[农业科学—农业机械化工程]