血管内皮生长因子和骨形态发生蛋白7在细胞分化中的协同作用  

Effect of recombinant human vascular endothelial growth factor and recombinant human bone morphogenetic protein-7 on the differentiation of rat osteoblast

作　　者：吴纪程[1] 兰泽栋[1,2] 张端强[1] 

机构地区：[1]福建医科大学附属口腔医学院,福建福州350002 [2]广州医学院口腔医院,广东广州510280

出　　处：《中国美容医学》2013年第19期1938-1941,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:研究rhVEGF和rhBMP-7对成骨细胞分化能力的影响。方法:取大鼠颅盖骨组织块,采用改良组织块混合酶消化法培养成骨细胞,将不同浓度的rhVEGF和rhBMP-7加入第四代成骨细胞的培养基继续进行细胞培养,用碱性磷酸酶试剂盒检测定细胞在第1、3、5天的磷酸酶的活性,分析不同浓度的rhVEGF和rhBMP-7对成骨细胞分化的影响。结果:rhVEGF和rhBMP-7能促进成骨细胞的分化,其中,3.125ng/ml rhVEGF和50.000ng/ml rhBMP-7单独或者两者联合作用并于第3天时,对成骨细胞碱性磷酸酶活性的影响最明显。结论:一定剂量的rhVEGF和rhBMP-7可明显促进成骨细胞的分化。Objective To study the effect of recombinant human vascular endothelial growth factor and recombinant human bone morphogenetic protein-7 on the differentiation of osteoblasts.Methods Cell culturing,Osteoblasts were cultivated with rhVEGF and rhBMP-7 of different concentrations for1,3,5 days,PNPP assay were used to observe the effect on differentiation in the cell morphology and the cellular cytomety of differentiation.Results rhVEGF and rhBMP-7 could stimulate osteoblasts differentiation.The maximal effect on differentiation was at a concentration of 3.125ng/m rhVEGF and 50.000ng/ml rhBMP-7 and in the third day.Conclusion rhVEGF and rhBMP-7 may stimulate bone formation in a direct fashion by induction of differentiation of osteoblasts.

关 键 词：重组人血管内皮因子 重组人骨形态发生蛋白-7 成骨细胞 分化 

分 类 号：Q813.1[生物学—生物工程]