1064nm Nd:YAG激光对5种甲真菌病重要致病菌的作用  

Effects of 1064nm Nd: YAG laser irradiation on 5 main pathogenic fungi of onychomycosis

作　　者：陈丽华[1,2] 朱红梅[1,2] 皇幼明[1,2] 胡禅[1,2] 谭宏月[1,2] 钟彬[1,2] 李平[1,2] 温海[1,2] 

机构地区：[1]上海长征医院皮肤病与真菌病研究所 全军真菌病重点实验室 [2]第二军医大学附属长征医院皮肤科,上海200003

出　　处：《中国真菌学杂志》2013年第4期205-209,共5页Chinese Journal of Mycology

摘　　要：目的观察1064nm Nd:YAG激光对5种甲真菌病重要致病菌(红色毛癣菌、须癣毛癣菌、白念珠菌、近平滑念珠菌、烟曲霉)生长的影响。方法将各菌的菌悬液均匀点种于培养基上,生长至形成一定大小的菌落,给予不同能量1064nm Nd:YAG激光照射,皮肤癣菌类观察激光照射后第1、3、6天菌落生长受抑制的直径变化情况;念珠菌类观察其次代培养存活率的差异;烟曲霉菌悬液铺满整个平皿,观察经不同能量激光照射受抑制的菌落空白处直径大小差异。结果在体外,当激光能量累计达到3 200 J/cm2时,可对培养基上生长的以上各菌产生生长抑制作用,且随着能量增大,抑制作用增强,甚至可发生杀灭作用。结论 1064nm Nd:YAG激光当能量累计达到一定量时可对甲真菌病致病菌产生明显的生长抑制或杀灭作用。Objective To observe the effect of 1064nm Nd： YAG laser irradiation on the growth of 5 main pathogenic fungi of on- ychomycosis including Trichophyton rubrum, Trichophyton mentagrophytes, Candida albicans, Candida parapsiZosis, Aspergillus fumigatus . Methods Suspension of T. rubrum , T. mentagrophytes , C. albicans , C. parapsilosis were spotted on ager media. After incubation for several days, the colonies were exposed to various energies of 1064nm Nd：YAG laser. For T. r andT. m , diameters of colonies were measured on the first, third, and sixth days after laser irradiation. For C. a and C. p , the subculture＇s survival rates of different energies of laser irradiation were assessed respectively. As for A.fumigatus , having been incubated on the whole plate, to Compare the different diameters of circular growth-free zones after different energies of laser treatment. Result In vitro , when the laser energy reaching 3 200 J/cm2, the growth of five pathogenic fungi of onychomycosis could be inhibited significantly. And the higher the laser energy was, the more significant the inhibition would be, and even pathogenic fungi would be killed. Conclusion When the energy of 1064nm Nd：YAG laser reaching a certain amount, the pathogenic fungi of onychomycosis could be inhibited sig- nificantly or be killed.

关 键 词：甲真菌病 皮肤癣菌 念珠菌 非皮肤癣菌性丝状真菌 1064nm Nd YAG激光 

分 类 号：R756.4[医药卫生—皮肤病学与性病学]