米格列奈对人主动脉内皮细胞NO合成及NOS活力的影响  

Effects of mitiglinide on nitric oxide production and synthase activity in human aortic endothelial cells

作　　者：蒋曦媛[1] 陆玉凤[1] 刘丽燕[1] 孙子林[2] 

机构地区：[1]南京中医药大学附属昆山市中医医院内分泌科,江苏省215300 [2]东南大学附属中大医院内分泌科

出　　处：《江苏医药》2013年第20期2386-2389,共4页Jiangsu Medical Journal

基　　金：昆山市社会发展科技计划项目(KS0912)

摘　　要：目的观察米格列奈对高糖环境下人主动脉内皮细胞(HAECs)一氧化氮(NO)合成及一氧化氮合酶(NOS)活力的影响。方法将培养的HAECs分为对照组(葡萄糖5.5mmol/L)、高糖组(葡萄糖30mmol/L)和米格列奈组(葡萄糖30mmol/L+米格列奈10μmol/L)。ELISA法检测NO含量及裂解液中内皮型NOS(eNOS)及诱导型NOS(iNOS)的含量,RT-PCR和Western blot分别检测细胞中eNOS mRNA和蛋白的表达。结果与对照组相比,高糖组24hNO含量升高(P<0.05),48、72h含量降低(P<0.05)。与高糖组相比,米格列奈组24hNO含量降低(P<0.05),48、72h含量升高(P<0.05)。与高糖组相比,米格列奈组iNOS含量降低(P<0.05),eNOS mRNA及蛋白表达升高(P<0.05)。结论米格列奈能减少高糖环境中HAECs的iNOS生成,增强eNOS活性和表达,促进内皮细胞NO产生。Objective To study the effects of mitiglinide on nitric oxide（NO） production and nitric oxide synthase （NOS） activity in human aortic endothelial cells （HAECs）. Methods HAECs were cultured and incubated with glucose 30 mmol/L（group A）, glucose 30 mmol/L＋mitiglinide 10/lmol/L（group B） and glucose 5.5 mmol/L（group C）, respectively. Cell lysate was collected and the contents of NO, endothelial nitric oxide synthase （eNOS） and inducible nitric oxide synthase （iNOS） were measured by ELISA. The expressions of eNOS mRNA and protein were analyzed by RT-PCR and Western blot, respectively. Results Compared with group C, the content of NO was increased at 24 hours and decreased at 48 hours and 72 hours after incubation in group A（P〈0. 05）. Compared with group A, the content of NO was decreased at 24 hours and increased at 48 hours and 72 hours after incubation in group B（P〈0. 05）. Compared with group A, the content of iNOS was decreased and the expressions of eNOS mRNA and protein were increased in group B（P〈0. 05）. Conclusion Mitiglinide is able to decrease iNOS activity, increase eNOS activity and its expression, and improve NO production in cultured HAECs with high glucose.

关 键 词：米格列奈 人主动脉内皮细胞 一氧化氮 一氧化氮合酶 高糖 

分 类 号：R587[医药卫生—内分泌]