三叶青提取物对肺癌A549细胞中Caspase-3表达的影响  

Effect of Clover extract on Caspase-3 expression in A549 lung carcinoma cells

作　　者：曾娟[1] 周婷[1] 童雪晴[1] 刘燕丽[1] 李遥[1] 王小莉[2] 

机构地区：[1]吉首大学医学院 [2]吉首大学医学院病理学与病理生理学教研室,湖南省416000

出　　处：《江苏医药》2013年第20期2401-2402,共2页Jiangsu Medical Journal

基　　金：湖南省大学生研究性学习和创新性实验计划[湘教通(2010)224号];吉首大学大学生研究性学习和创新性实验计划(2010-36)

摘　　要：目的探讨三叶青提取物对肺癌A549细胞半胱天冬氨酸蛋白酶3(Caspase-3)表达的影响。方法分别用浓度为0.1、0.01和0.001g/L的三叶青提取物(D1、D2和D3组)和生理盐水(对照组)处理体外培养的肺癌A549细胞48h后,采用Western blot法检测各组细胞中Caspase-3的表达。结果 D1、D2和D3组Caspase-3的表达均高于C组(P<0.05),并且呈浓度依赖性。结论三叶青提取物可通过激活Caspase-3诱导人肺癌A549细胞凋亡,并且呈剂量依赖性。Objective To study the effect of Clover extract on expression of cysteine-aspartic acid protease 3 （Caspase-3） in A549 lung carcinoma cells. Methods A549 lung carcinoma cells cultured in vitro were divided into four groups and treated with different concentrations of Clover extract 0. 1,0.01 and 0. 001 g/L（groups of D1,D2 and D3） or normal saline（group C） for 48 hours. The A549 cells were collected and lysed for detecting Caspase-3 expression using Western blot. Results Caspase-3 expression in A549 cells was induced by Clover extract in a concentration-dependent manner,which was higher in groups of D1, D2 and D3 than that in group C（P^0. 05）. Conclusion The Clover extract induces apoptosis of A549 lung carcinoma cells through activating Caspase-3 pathway in a dose-dependent manner.

关 键 词：三叶青提取物 A549细胞 半胱天冬氨酸蛋白酶3 

分 类 号：R285[医药卫生—中药学]