肾移植受者移植前免疫细胞亚群的检测及意义  

Detection of immune cell subsets in renal allograft recipients before operation and its significance

作　　者：黎东伟[1] 刘龙山[1] 费继光[1] 王长希[1] 

机构地区：[1]中山大学附属第一医院器官移植中心,广东省广州市510080

出　　处：《中国组织工程研究》2013年第44期7675-7680,共6页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技计划项目(2009B030801145)~~

摘　　要：背景:对肾移植受者免疫细胞的研究一直是移植外科的研究热点,但国内外缺少对肾移植受者术前全面、系统的免疫检测研究。目的:探讨肾移植受者术前免疫细胞亚群的比例分布。方法:纳入准备接受初次活体肾移植受者共15例作实验组;以18-40岁健康志愿者15人作对照组,分别抽取外周静脉血进行流式细胞检测。结果与结论:T细胞亚群:肾移植受者CD4+CD25+T细胞占外周血淋巴细胞的比例、CD4+CD25+T细胞占CD4+T细胞的比例均低于健康人群,差异有显著性意义(P<0.05),其余细胞亚群比例差异无显著性意义。B细胞亚群:肾移植受者CD19+/淋巴细胞、CD19+CD5+/淋巴细胞、CD19+CD27+/淋巴细胞均低于健康者,而CD38+IgD-/CD19+高于健康者,差异有显著性意义(P<0.05),其他B细胞亚群比较差异无显著性意义。NK及NKT细胞亚群的比较:肾移植受者NKG2A/NK细胞、NKG2A/NKG2D均低于健康者、而NKG2D/细胞显著高于健康者(P<0.05),NK/淋巴细胞、NKT/淋巴细胞差异无显著性意义。说明肾移植受者移植前进行免疫细胞亚群流式分析,可以了解受者移植前免疫状态,对移植后免疫监测起基础对照作用。BACKGROUND：The immune cells of renal al ograft recipients have always been the hot spot of research. However, there are few studies addressing the immune cellsubsets in renal al ograft recipients before operation. OBJECTIVE：To investigate the proportional distribution of immune cellsubsets in renal al ograft recipients before operation. METHODS：Fifteen de novo living-related renal transplant recipients were enrol ed in this study with 15 healthy volunteers, aged 18-40 years, as healthy controls. Flow cytometry was employed to observe the proportion of the immune cellsubsets by extracting peripheral venous blood of al participants. RESULTS AND CONCLUSION：In the renal al ograft recipients, the proportions of CD4＋CD25＋T cells, the proportion of CD4＋CD25＋/CD4＋T cells, CD19＋B cells, CD19＋CD5＋B cells, CD19＋CD27＋B cells, NKG2A/NK cells, and NKG2A/NKG2 cells were al lower than those in the healthy controls;however, the proportion of CD38＋IgD-/CD19＋B cells and NKG2D cells were higher than those in the healthy controls. The difference of the proportion of immune cellsubsets aforementioned between the two groups was statistical y significant （P〈0.05）, while no difference was observed in other subsets. Immune cellsubsets in renal al ograft recipients before operation could be used to assess the immune status of the recipients, and also could be seen as the basal control for postoperative immunological monitoring.

关 键 词：器官移植 肾移植 尿毒症 免疫细胞亚群 移植免疫 免疫检测 移植排斥 省级基金 

分 类 号：R617[医药卫生—外科学]